| Title: |
P1332 Back to the future: high-throughput culturomics reveals mechanistic insights into hydrogen sulphide production in ulcerative colitis |
| Authors: |
Raja, S; Costello, S; Rayner, C; Wheeler, R; Uylaki, W; Young, R; Forster, S; Day, A; Bibb, L; Bryant, R V |
| Source: |
Journal of Crohn’s and Colitis ; volume 20, issue Supplement_1 ; ISSN 1873-9946 1876-4479 |
| Publisher Information: |
Oxford University Press (OUP) |
| Publication Year: |
2026 |
| Description: |
Background Gut microbiota perturbations are linked to ulcerative colitis (UC), but mechanistic data tying specific microbial functions to disease activity are limited. Current insights largely derive from culture independent sequencing methods which cannot distinguish viable from non-viable organisms and provide only inferred, rather than experimentally verified, functional potential. High throughput culturomics combines anaerobic culturing with sequencing to recover viable, and disease-associated isolates that permit direct phenotypic testing. Hydrogen sulphide (H2S) is a microbial by-product implicated in UC through direct epithelial injury and inhibition of colonocyte butyrate utilisation. We applied culturomics to patients with ulcerative proctitis (UP) enrolled in the UP-FMT trial to i) identify isolates associated with active disease in vivo and ii) map the taxonomic distribution of H2S production Methods Baseline stool and rectal mucosal biopsies from patients with active ulcerative proctitis in the UP-FMT pilot study were homogenised and cultured anaerobically on a variety of bacteriological media. After incubation, individual colonies were subcultured in broth and used as template for full-length 16S rRNA PCR and sequencing for taxonomic assignment. Isolates were phenotyped for H2S production from organic (L-cysteine) and inorganic (thiosulphate) sulphur sources, and phylogenetic patterns in this trait were explored. Results Baseline stool and/or mucosal samples were collected from 13 patients. In total 7,125 isolates were obtained with culturing, yielding 5,192 high-quality full-length 16S sequences and 380 species-level OTUs. We identified 141 putative novel species (104 from stool, 61 from mucosa). Multiple clades, particularly within Bacillota and Bacteroidata, consisted exclusively of mucosa-derived isolates. H2S production was widely distributed across diverse taxa, spanning Bacillota, Bacteroidata, Eggerthela related clades (actinomycetota) and Escherichia-related clades ... |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| DOI: |
10.1093/ecco-jcc/jjaf231.1513 |
| Availability: |
https://doi.org/10.1093/ecco-jcc/jjaf231.1513; https://academic.oup.com/ecco-jcc/article-pdf/20/Supplement_1/jjaf231.1513/66502741/jjaf231.1513.pdf |
| Rights: |
https://academic.oup.com/pages/standard-publication-reuse-rights |
| Accession Number: |
edsbas.C1AAC2D6 |
| Database: |
BASE |