| Title: |
Construction of a Multitissue Cell Atlas Reveals Cell-Type-Specific Regulation of Molecular and Complex Phenotypes in Pigs |
| Authors: |
Chen, Lijuan; Li, Houcheng; Teng, Jinyan; Wang, Zhen; Qu, Xiaolu; Chen, Zhe; Cai, Xiaodian; Zeng, Haonan; Ballester Devis, Maria; Crespo-Piazuelo, Daniel |
| Contributors: |
Producció Animal; Genètica i Millora Animal |
| Publisher Information: |
Wiley |
| Publication Year: |
2025 |
| Collection: |
IRTA Pubpro (Institute of Agrifood Research and Technology / Institut de Recerca i Tecnologia Agroalimentàries) |
| Time: |
636 |
| Description: |
The systematic characterization of cellular heterogeneity among tissues and cell-type-specific regulation underlying complex phenotypes remains elusive in pigs. Within the Pig Genotype-Tissue Expression (PigGTEx) project, this work presents a single-cell transcriptome atlas of adult pigs encompassing 229 268 high-quality nuclei from 19 tissues, annotated to 67 major cell types. Besides cellular heterogeneity within and across tissues, this work further characterizes prominent tissue-specific features and functions of muscle, epithelial, and immune cells. Through deconvoluting 3921 bulk RNA-seq samples from 17 matching tissues, this work dissects thousands of genetic variants with cell-type interaction effects on gene expression (ieQTL). By colocalizing these ieQTL with variants associated with 268 complex traits, new insights into the cellular mechanisms behind these traits are provided. Moreover, this work highlights that orthologous genes with cell-type-specific regulation in pigs exhibit significant heritability enrichment for some human complex phenotypes. Altogether, this work provides a valuable resource and highlights novel insights in cellular regulation of complex traits for accelerating pig precision breeding and human biomedical research. ; The authors thank Prof. Elisabetta Giuffra (INRAE, France) for her valuable comments and suggestions on this project. This work was supported by the Agricultural Science and Technology Innovation Program of the Chinese Academy of Agricultural Sciences (CAAS-SCAB-202302), the National Key Research and Development Program of China (2021YFF1000600), the National Natural Science Foundation of China (32002150 to G.Y., 32022078 to Z.Z.), the Local Innovative and Research Teams Project of Guangdong Province (2019BT02N630 to Z.Z.), the China Agriculture Research System (CARS-35 to Z.Z. and J.L.), the Key Research and Development projects in Yangzhou City (YZ2021037 to Z.C.), the Independent Research Fund Denmark (8048-00072A to L.L.), the Novo Nordisk Foundation ... |
| Document Type: |
article in journal/newspaper |
| File Description: |
application/pdf |
| Language: |
English |
| Relation: |
Advanced Science; https://hdl.handle.net/20.500.12327/4893; https://doi.org/10.1002/advs.202504961 |
| DOI: |
10.1002/advs.202504961 |
| Availability: |
https://hdl.handle.net/20.500.12327/4893; https://doi.org/10.1002/advs.202504961 |
| Rights: |
Attribution 4.0 International ; http://creativecommons.org/licenses/by/4.0/ ; info:eu-repo/semantics/openAccess |
| Accession Number: |
edsbas.C1D4D3CF |
| Database: |
BASE |