| Title: |
Small RNA F6 Provides Mycobacterium smegmatis Entry into Dormancy |
| Authors: |
Artem Grigorov; Oksana Bychenko; Elena G. Salina; Yulia Skvortsova; Arina Mazurova; Timofey Skvortsov; Arseny Kaprelyants; Tatyana Azhikina |
| Source: |
International Journal of Molecular Sciences, Vol 22, Iss 11536, p 11536 (2021) |
| Publisher Information: |
MDPI AG |
| Publication Year: |
2021 |
| Collection: |
Directory of Open Access Journals: DOAJ Articles |
| Subject Terms: |
Mycobacterium smegmatis; small non-coding RNA; F6; resuscitation promoting factor RpfE2; dormancy; non-culturability; Biology (General); QH301-705.5; Chemistry; QD1-999 |
| Description: |
Regulatory small non-coding RNAs play a significant role in bacterial adaptation to changing environmental conditions. Various stresses such as hypoxia and nutrient starvation cause a reduction in the metabolic activity of Mycobacterium smegmatis , leading to entry into dormancy. We investigated the functional role of F6, a small RNA of M. smegmatis , and constructed an F6 deletion strain of M. smegmatis . Using the RNA-seq approach, we demonstrated that gene expression changes that accompany F6 deletion contributed to bacterial resistance against oxidative stress. We also found that F6 directly interacted with 5′-UTR of MSMEG_4640 mRNA encoding RpfE2, a resuscitation-promoting factor, which led to the downregulation of RpfE2 expression. The F6 deletion strain was characterized by the reduced ability to enter into dormancy (non-culturability) in the potassium deficiency model compared to the wild-type strain, indicating that F6 significantly contributes to bacterial adaptation to non-optimal growth conditions. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| Relation: |
https://www.mdpi.com/1422-0067/22/21/11536; https://doaj.org/toc/1661-6596; https://doaj.org/toc/1422-0067; https://doaj.org/article/bdfeabbca70143e9b7af5551b8ba91e9 |
| DOI: |
10.3390/ijms222111536 |
| Availability: |
https://doi.org/10.3390/ijms222111536; https://doaj.org/article/bdfeabbca70143e9b7af5551b8ba91e9 |
| Accession Number: |
edsbas.C21F6E22 |
| Database: |
BASE |