| Title: |
Human Leukocyte Antigen-G is enriched in presence of trypanosome in the dermis of individuals exposed to gambiense Human African Trypanosomiasis in Guinea and Côte d’Ivoire |
| Authors: |
Lagrave, Alisé; Camara, Aïssata; Gineau, Laure; Tichit, Magali; Gnankou, Firmin Bolivar; Soumah, Alseny M’mah; Camara, Mariame; N’djetchi, Martial; Kaboré, Justin Windingoudi; Camara, Oumou; Coulibaly, Bamoro; Sépé, Blé; Nanan, Valentin; Kouadio, Koffi Alain de Marie; N’dri, Louis; Konan, Thomas; Milet, Jacqueline; Boiro, Salimatou; Travaillé, Christelle; Crouzols, Aline; Petiot, Nathalie; Ilboudo, Hamidou; Hardy, David; Sadissou, Ibrahim; Bart, Jean-Mathieu; Camara, Mamadou; Kaba, Dramane; Koffi, Mathurin; Bucheton, Bruno; Jamonneau, Vincent; Courtin, David; Rotureau, Brice |
| Contributors: |
Mère et enfant en milieu tropical : pathogènes, système de santé et transition épidémiologique (MERIT - UMR_D 261); Institut de Recherche pour le Développement (IRD)-Université Paris Cité (UPCité); Institut Pasteur de Guinée; Pasteur Network (Réseau International des Instituts Pasteur); Plate-Forme d’Histopathologie / Histopathology Platform; Institut Pasteur Paris (IP)-Université Paris Cité (UPCité); Institut Pierre Richet Bouaké, Côte d'Ivoire (IPR); Institut national de santé publique Abidjan, Côte d'Ivoire (INSP (Côte d'Ivoire)); Ministère de la Santé Conakry, Guinea; Université Jean Lorougnon Guédé (UJloG); Biologie cellulaire des Trypanosomes - Trypanosome Cell Biology; Institut Pasteur Paris (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPCité); Institut Pasteur Paris (IP); Institut de Recherche en Sciences de la Santé Ouagadougou, Burkina Faso (IRSS); Institut de Recherche pour le Développement (IRD); Interactions hôtes-vecteurs-parasites-environnement dans les maladies tropicales négligées dues aux trypanosomatides (UMR INTERTRYP); Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut de Recherche pour le Développement (IRD)-Université de Montpellier (UM); This work was supported by the Institut Pasteur (DH and BR) and the Institut Pasteur of Guinea (BR), the Institut de Recherche pour le Développement (DEPIST-THA project to DC), the French Government Investissement d’Avenir programme - Laboratoire d’Excellence “Integrative Biology of Emerging Infectious Diseases” (ANR-10-LABX-62-IBEID to BR), the French National Agency for Scientific Research (project ANR-18-CE15-0012 TrypaDerm to BR), and the Bill and Melinda Gates Foundation (www.gatesfoundation.org) through the Trypa-NO! Project (grant number INV-001785 to JMB, BB and VJ). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.; We warmly thank the team of the Programme National de Lutte contre la Trypanosomiase Humaine Africaine of Guinea, as well as all our collaborators of the Forecariah and Boffa Health Districts. We warmly thank the team of the Programme National d’Elimination de la Trypanosomiase Humaine Africaine of Côte d’Ivoire, as well as all our collaborators of the Sinfra and Bouaflé Health Districts. We thank M. Carrington (University of Cambridge, UK) for providing the anti-ISG65 antibody, and Nadine Fievet who provided the human placenta sections used as positive control; ANR-18-CE15-0012,TrypaDerm,Comprendre la biologie des trypanosomes africains dans la peau(2018); ANR-10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases(2010) |
| Source: |
ISSN: 1935-2727. |
| Publisher Information: |
CCSD; Public Library of Science |
| Publication Year: |
2026 |
| Subject Terms: |
[SDV]Life Sciences [q-bio] |
| Description: |
International audience ; Human leukocyte antigen-G (HLA-G) is an immunomodulatory molecule known to play a crucial role in immune tolerance and regulation. In the context of human African trypanosomiasis (HAT), higher soluble HLA-G levels were detected in the plasma of confirmed cases, representing a serological marker of T. b. gambiense infection. As trypanosomes also invade extravascular tissues, especially the skin, this study explored the potential role of HLA-G in the dermal immune response during T. b. gambiense infection. Blood and skin samples from 50 seronegative individuals, 45 seropositive suspects and 36 confirmed HAT cases, collected between 2018 and 2022 in endemic foci of Guinea and Côte d’Ivoire, were analyzed. Plasmatic and dermal levels of HLA-G proteins were quantified by ELISA and immuno-histochemistry, respectively, and compared to the trypanosome detection results in the same samples. The implication of soluble HLA-G plasma level as a biomarker of T. b. gambiense infection was confirmed. In the dermis, HLA-G isoforms were expressed either with a granular distribution or with in diffuse halos. Granular patterns of dermal HLA-G were directly associated with the presence of trypanosomes in the dermis. The presence of diffuse halos was correlated to higher sHLA-G levels in the plasma. In total, this study provides the first evidence of the involvement of HLA-G in the extravascular immune response against parasites, especially in the skin. It shows that HLA-G distribution in the extravascular compartment also represents a biomarker of trypanosome infection. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| Relation: |
info:eu-repo/semantics/altIdentifier/pmid/41802004; https://pasteur.hal.science/view/index/docid/5545739; https://pasteur.hal.science/view/index/docid/5545739/document; https://pasteur.hal.science/view/index/docid/5545739/file/journal.pntd.0014085.pdf; PUBMED: 41802004; PUBMEDCENTRAL: PMC12987593 |
| DOI: |
10.1371/journal.pntd.0014085 |
| Availability: |
https://pasteur.hal.science/view/index/docid/5545739; https://pasteur.hal.science/view/index/docid/5545739/document; https://pasteur.hal.science/view/index/docid/5545739/file/journal.pntd.0014085.pdf; https://doi.org/10.1371/journal.pntd.0014085 |
| Rights: |
https://creativecommons.org/licenses/by/4.0/ ; info:eu-repo/semantics/OpenAccess |
| Accession Number: |
edsbas.C23C6041 |
| Database: |
BASE |