| Title: |
Matrix metalloproteinase-9 activity detected in body fluids is the result of two different enzyme forms |
| Authors: |
Bellini, Tiziana; Trentini, Alessandro; Manfrinato, Maria C.; Tamborino, Carmine; Volta, Carlo Alberto; Di Foggia, Valentina; Fainardi, Enrico; Dallocchio, Franco; Castellazzi, Massimiliano |
| Publisher Information: |
Oxford University Press |
| Publication Year: |
2012 |
| Collection: |
HighWire Press (Stanford University) |
| Subject Terms: |
Regular Paper |
| Description: |
In vitro activation of Matrix metalloproteinase-9 (MMP-9) (gelatinase B) with MMP-3 shows the presence of two different forms: an 82 kDa, N-terminal truncated form, and a 65 kDa, N- and Cterminal truncated form. So far the presence of the 65 kDa form has not been reported in vivo. Affinity chromatography was performed to separate MMP-9 from MMP-2 and immunoprecipitation to isolate ∼65 kDa MMP-9 from 82 kDa MMP-9 in sera of healthy donors. The presence of ∼65 kDa active MMP-9 was demonstrated both with gelatin zymography and western blot analysis. The ∼65 kDa MMP-9 lacks the hemopexin domain required for the high affinity binding of the tissue inhibitor TIMP-1, and can be evaluated by activity assay in the presence of TIMP-1. This opens the possibility to investigate the role of this form of MMP-9 that escapes physiological regulation. |
| Document Type: |
text |
| File Description: |
text/html |
| Language: |
English |
| Relation: |
http://jb.oxfordjournals.org/cgi/content/short/mvs014v1; http://dx.doi.org/10.1093/jb/mvs014 |
| DOI: |
10.1093/jb/mvs014 |
| Availability: |
http://jb.oxfordjournals.org/cgi/content/short/mvs014v1; https://doi.org/10.1093/jb/mvs014 |
| Rights: |
Copyright (C) 2012, Japanese Biochemical Society |
| Accession Number: |
edsbas.C28558D2 |
| Database: |
BASE |