| Title: |
Synthetic self-assembling ADDomer platform for highly efficient vaccination by genetically encoded multiepitope display |
| Authors: |
Vragniau, Charles; Bufton, Joshua; Garzoni, Frédéric; Stermann, Emilie; Rabi, Fruzsina; Terrat, Céline; Guidetti, Mélanie; Josserand, Véronique; Williams, Matt; Woods, Christopher; Viedma, Gerardo; Bates, Phil; Verrier, Bernard; Chaperot, Laurence; Schaffitzel, Christiane; Berger, Imre; Fender, Pascal |
| Contributors: |
Institut de biologie structurale (IBS - UMR 5075); Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes 2016-2019 (UGA 2016-2019 )-Institut de Recherche Interdisciplinaire de Grenoble (IRIG); Direction de Recherche Fondamentale (CEA) (DRF (CEA)); Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)); Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA); University of Bristol Bristol; Imophoron Ltd; Laboratoire de Biologie Tissulaire et d'ingénierie Thérapeutique (LBTI); Université Claude Bernard Lyon 1 (UCBL); Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS); Institute for Advanced Biosciences / Institut pour l'Avancée des Biosciences (Grenoble) (IAB); Établissement Français du Sang Auvergne-Rhône-Alpes Lyon (EFS Auvergne-Rhône-Alpes - Lyon); Établissement Français du Sang La Plaine Saint-Denis (EFS)-Établissement Français du Sang La Plaine Saint-Denis (EFS)-CHU de Grenoble-Alpes - Centre Hospitalier Universitaire CHU Grenoble (CHUGA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes 2016-2019 (UGA 2016-2019 ); Oracle Cloud Development Centre; Institut de biologie et chimie des protéines Lyon (IBCP); Max Planck-Bristol Centre for Minimal Biology; ANR-10-INBS-0005,FRISBI,Infrastructure Française pour la Biologie Structurale Intégrée(2010); ANR-10-LABX-0049,GRAL,Grenoble Alliance for Integrated Structural Cell Biology(2010) |
| Source: |
ISSN: 2375-2548 ; Science Advances ; https://hal.science/hal-02333174 ; Science Advances , 2019, 5 (9), pp.eaaw2853. ⟨10.1126/sciadv.aaw2853⟩. |
| Publisher Information: |
CCSD; American Association for the Advancement of Science (AAAS) |
| Publication Year: |
2019 |
| Collection: |
HAL Lyon 1 (University Claude Bernard Lyon 1) |
| Subject Terms: |
[SDV.IMM.VAC]Life Sciences [q-bio]/Immunology/Vaccinology; [SDV.BIO]Life Sciences [q-bio]/Biotechnology |
| Description: |
International audience ; Self-assembling virus-like particles represent highly attractive tools for developing next-generation vaccines and protein therapeutics. We created ADDomer, an adenovirus-derived multimeric protein-based self-assembling nanoparticle scaffold engineered to facilitate plug-and-play display of multiple immunogenic epitopes from pathogens. We used cryo-electron microscopy at near-atomic resolution and implemented novel, cost-effective, high-performance cloud computing to reveal architectural features in unprecedented detail. We analyzed ADDomer interaction with components of the immune system and developed a promising first-in-kind ADDomer-based vaccine candidate to combat emerging Chikungunya infectious disease, exemplifying the potential of our approach. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| Relation: |
info:eu-repo/semantics/altIdentifier/pmid/31620562; PUBMED: 31620562; PUBMEDCENTRAL: PMC6763337 |
| DOI: |
10.1126/sciadv.aaw2853 |
| Availability: |
https://hal.science/hal-02333174; https://hal.science/hal-02333174v1/document; https://hal.science/hal-02333174v1/file/eaaw2853.full.pdf; https://doi.org/10.1126/sciadv.aaw2853 |
| Rights: |
https://about.hal.science/hal-authorisation-v1/ ; info:eu-repo/semantics/OpenAccess |
| Accession Number: |
edsbas.C371661C |
| Database: |
BASE |