| Title: |
Diagnostic Accuracy of Microscopic Observation Drug Susceptibility (MODS) Assay for Pediatric Tuberculosis in Hanoi, Vietnam |
| Authors: |
Tran, ST; Renschler, JP; Le, HT; Dang, HTT; Dao, TM; Pham, AN; Nguyen, LT; van Nguyen, H; Thi Thu Nguyen, T; Ngoc Le, S; Fox, A; Caws, M; Thi Quynh, ND; Horby, P; Wertheim, H |
| Contributors: |
Connell, TG |
| Publisher Information: |
PUBLIC LIBRARY SCIENCE |
| Publication Year: |
2013 |
| Collection: |
The University of Melbourne: Digital Repository |
| Description: |
INTRODUCTION: Microscopic [corrected] Observation Drug Susceptibility (MODS) has been shown to be an effective and rapid technique for early diagnosis of tuberculosis (TB). Thus far only a limited number of studies evaluating MODS have been performed in children and in extra-pulmonary tuberculosis. This study aims to assess relative accuracy and time to positive culture of MODS for TB diagnosis in children admitted to a general pediatric hospital in Vietnam. METHODS/PRINCIPAL FINDINGS: Specimens from children with suspected TB were tested by smear, MODS and Lowenstein-Jensen agar (LJ). 1129 samples from 705 children were analyzed, including sputum (n=59), gastric aspirate (n=775), CSF (n=148), pleural fluid (n=33), BAL (n=41), tracheal fluid (n=45), other (n=28). 113 TB cases were defined based on the "clinical diagnosis" (confirmed and probable groups) as the reference standard, in which 26% (n=30) were diagnosed as extra-pulmonary TB. Analysis by patient shows that the overall sensitivity and specificity of smear, LJ and MODS against "clinical diagnosis" was 8.8% and 100%, 38.9% and 100%, 46% and 99.5% respectively with MODS significantly more sensitive than LJ culture (P=0.02). When analyzed by sample type, the sensitivity of MODS was significantly higher than LJ for gastric aspirates (P=0.004). The time to detection was also significantly shorter for MODS than LJ (7 days versus 32 days, P |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| ISSN: |
1932-6203 |
| Relation: |
Tran, S. T., Renschler, J. P., Le, H. T., Dang, H. T. T., Dao, T. M., Pham, A. N., Nguyen, L. T., van Nguyen, H., Thi Thu Nguyen, T., Ngoc Le, S., Fox, A., Caws, M., Thi Quynh, N. D., Horby, P. & Wertheim, H. (2013). Diagnostic Accuracy of Microscopic Observation Drug Susceptibility (MODS) Assay for Pediatric Tuberculosis in Hanoi, Vietnam. Plos One, 8 (9), https://doi.org/10.1371/journal.pone.0072100.; https://hdl.handle.net/11343/262917 |
| Availability: |
https://hdl.handle.net/11343/262917 |
| Rights: |
https://creativecommons.org/licenses/by/4.0 ; CC BY |
| Accession Number: |
edsbas.C387A50F |
| Database: |
BASE |