| Title: |
Interleukin 34 exerts anti-inflammatory activities on keratinocytes and is down-regulated in psoriatic-inflamed skin |
| Authors: |
Croquette, Magali; Faugeroux, Alicia; Fonlupt, Clémence; Godet, Julie; Frouin, Éric; Garcia, Martine; Bernard, François-Xavier; Cordier-Dirikoc, Sevda; Pedretti, Nathalie; Larid, Guillaume; Favot, Laure; Roblot, Pascal; Boutin, Damien; Hainaut-Wierzbicka, Ewa; Heymann, Dominique; Lecron, Jean-Claude; Morel, Franck; Jégou, Jean-François |
| Contributors: |
Laboratoire inflammation, tissus épithéliaux et cytokines UR 15560 (LITEC Poitiers ); Université de Poitiers = University of Poitiers (UP); Service de Médecine Interne, Maladies Infectieuses et Tropicales CHU Poitiers; Centre hospitalier universitaire de Poitiers = Poitiers University Hospital (CHU de Poitiers La Milétrie ); Service de Chirurgie Vasculaire Poitiers; QIMA Bioalternatives (QIMA Life Sciences); Médecine Interne et Maladies Infectieuses, CHU de Poitiers, Poitiers, France; Service d’Anatomo-Pathologie et Pathologie ultrastructurale CHU Poitiers; Service de rhumatologie Poitiers; Département de Dermatologie CHU Poitiers; Nantes Université (Nantes Univ); Institut de Cancérologie de l'Ouest Angers/Nantes (UNICANCER/ICO); UNICANCER; Service Immunologie/Inflammation CHU Poitiers; This study was supported by grants from AbbVie, the University of Poitiers and the “Association des Sclérodermiques de France”. We thank the Région Nouvelle-Aquitaine and the European Union for equipment grant program for research laboratories and platforms. |
| Source: |
ISSN: 0022-202X. |
| Publisher Information: |
CCSD; Nature Publishing Group |
| Publication Year: |
2023 |
| Collection: |
Université de Nantes: HAL-UNIV-NANTES |
| Subject Terms: |
Interleukin 34; cytokine; keratinocytes; skin inflammation; psoriasis; [SDV.IMM]Life Sciences [q-bio]/Immunology; [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology |
| Description: |
International audience ; Although interleukin-34 (IL-34) expression has been originally reported in the skin to play a role in Langerhans cell (LC) homeostasis, less is known about its contribution to skin inflammation. Here, we have investigated the cutaneous expression, regulation and role of IL-34 in psoriasis, as well as the expression of its receptors CSF-1R, PTPRZ1 and Syndecan-1. We showed that IL-34 expression is markedly decreased in psoriatic skin compared to healthy skin and restored after a successful anti-TNFα treatment. Consistent with its role in the maintenance of LC within the epidermis, the lowered expression of IL-34 was associated with a reduced density of LC. We identified a correlation between IL-34 and cytokeratin 10 or filaggrin expressions, suggesting a link with epidermal differentiation. In vitro experiments using primary keratinocytes cultured in monolayer or differentiated into reconstituted human epidermis confirmed that IL-34 expression is associated to the level of epidermal differentiation. When exposed to proinflammatory cytokines, keratinocytes underwent IL-34 downregulation. Finally, we demonstrated that IL-34 counteracts the proinflammatory effects of oncostatin M on epidermal differentiation by modulating STAT3 signaling pathway. In conclusion, IL-34 has regulatory properties in the skin by directly targeting keratinocytes and is downregulated by proinflammatory cytokines in inflamed skin during psoriasis. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| DOI: |
10.1016/j.jid.2023.05.023 |
| Availability: |
https://univ-poitiers.hal.science/hal-04133828; https://univ-poitiers.hal.science/hal-04133828v1/document; https://univ-poitiers.hal.science/hal-04133828v1/file/JID-2022-0251-Article%20merged.pdf; https://doi.org/10.1016/j.jid.2023.05.023 |
| Rights: |
https://about.hal.science/hal-authorisation-v1/ ; info:eu-repo/semantics/OpenAccess |
| Accession Number: |
edsbas.C3E009FC |
| Database: |
BASE |