| Title: |
Syncrip/hnRNP Q is required for activity-induced Msp300/Nesprin-1 expression and new synapse formation |
| Authors: |
Titlow, Joshua; Robertson, Francesca; Järvelin, Aino; Ish-Horowicz, David; Smith, Carlas; Gratton, Enrico; Davis, Ilan |
| Publisher Information: |
Rockefeller University Press |
| Publication Year: |
2020 |
| Collection: |
University of Glasgow: Enlighten - Publications |
| Description: |
Memory and learning involve activity-driven expression of proteins and cytoskeletal reorganization at new synapses, requiring posttranscriptional regulation of localized mRNA a long distance from corresponding nuclei. A key factor expressed early in synapse formation is Msp300/Nesprin-1, which organizes actin filaments around the new synapse. How Msp300 expression is regulated during synaptic plasticity is poorly understood. Here, we show that activity-dependent accumulation of Msp300 in the postsynaptic compartment of theDrosophila larval neuromuscular junction is regulated by the conserved RNA binding protein Syncrip/hnRNP Q. Syncrip (Syp) binds tomsp300 transcripts and is essential for plasticity. Single-molecule imaging shows that msp300 is associated with Syp in vivo and forms ribosome-rich granules that contain the translation factor eIF4E. Elevated neural activity alters the dynamics of Syp and the number ofmsp300:Syp:eIF4E RNP granules at the synapse, suggesting that these particles facilitate translation. These results introduce Syp as an important early acting activity-dependent regulator of a plasticity gene that is strongly associated with human ataxias. |
| Document Type: |
article in journal/newspaper |
| File Description: |
text |
| Language: |
English |
| Relation: |
https://eprints.gla.ac.uk/340109/1/340109.pdf; Titlow, Joshua, Robertson, Francesca, Järvelin, Aino, Ish-Horowicz, David, Smith, Carlas, Gratton, Enrico and Davis, Ilan ORCID logoorcid:0000-0002-5385-3053 (2020) Syncrip/hnRNP Q is required for activity-induced Msp300/Nesprin-1 expression and new synapse formation. Journal of Cell Biology , 219(3), e201903135. (doi:10.1083/jcb.201903135 ) (PMID:32040548) (PMCID:PMC7055005) |
| DOI: |
10.1083/jcb.201903135 |
| Availability: |
https://eprints.gla.ac.uk/340109/; https://eprints.gla.ac.uk/340109/1/340109.pdf; https://doi.org/10.1083/jcb.201903135 |
| Rights: |
cc_by_4 |
| Accession Number: |
edsbas.C4369D27 |
| Database: |
BASE |