| Title: |
Distinct gene expression dynamics in developing and regenerating crustacean limbs |
| Authors: |
Sinigaglia, Chiara; Almazán, Alba; Lebel, Marie; Sémon, Marie; Gillet, Benjamin; Hughes, Sandrine; Edsinger, Eric; Averof, M.; Paris, Mathilde |
| Contributors: |
Institut de Génomique Fonctionnelle de Lyon (IGFL); École normale supérieure de Lyon (ENS de Lyon); Université de Lyon-Université de Lyon-Université Claude Bernard Lyon 1 (UCBL); Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE); Laboratoire de biologie et modélisation de la cellule (LBMC UMR 5239); Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS); The Salk Institute for Biological Studies |
| Source: |
ISSN: 0027-8424. |
| Publisher Information: |
CCSD; National Academy of Sciences |
| Publication Year: |
2022 |
| Collection: |
HAL Lyon 1 (University Claude Bernard Lyon 1) |
| Subject Terms: |
transcriptional profiling; crustacean; leg development; regeneration; Parhyale hawaiensis; MESH: Amphipoda; MESH: Animals; MESH: Embryo; Nonmammalian; MESH: Extremities; MESH: Gene Expression; MESH: Regeneration; [SDV.BDD]Life Sciences [q-bio]/Development Biology |
| Description: |
International audience ; Regenerating animals have the ability to reproduce body parts that were originally made in the embryo and subsequently lost due to injury. Understanding whether regeneration mirrors development is an open question in most regenerative species. Here, we take a transcriptomics approach to examine whether leg regeneration shows similar temporal patterns of gene expression as leg development in the embryo, in the crustacean Parhyale hawaiensis . We find that leg development in the embryo shows stereotypic temporal patterns of gene expression. In contrast, the dynamics of gene expression during leg regeneration show a higher degree of variation related to the physiology of individual animals. A major driver of this variation is the molting cycle. We dissect the transcriptional signals of individual physiology and regeneration to obtain clearer temporal signals marking distinct phases of leg regeneration. Comparing the transcriptional dynamics of development and regeneration we find that, although the two processes use similar sets of genes, the temporal patterns in which these genes are deployed are different and cannot be systematically aligned. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| Relation: |
info:eu-repo/semantics/altIdentifier/pmid/35776546; PUBMED: 35776546; PUBMEDCENTRAL: PMC9271199 |
| DOI: |
10.1073/pnas.2119297119 |
| Availability: |
https://cnrs.hal.science/hal-03799485; https://cnrs.hal.science/hal-03799485v1/document; https://cnrs.hal.science/hal-03799485v1/file/Sinigaglia_revised_2.pdf; https://doi.org/10.1073/pnas.2119297119 |
| Rights: |
https://about.hal.science/hal-authorisation-v1/ ; info:eu-repo/semantics/OpenAccess |
| Accession Number: |
edsbas.C4EAF59C |
| Database: |
BASE |