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Low-cost assembly of a cacao crop genome is able to resolve complex heterozygous bubbles

Title: Low-cost assembly of a cacao crop genome is able to resolve complex heterozygous bubbles
Authors: Joe Morrissey; J. Conrad Stack; Rebecca Valls; Juan Carlos Motamayor
Source: Horticulture Research, Vol 6, Iss 1, Pp 1-13 (2019)
Publisher Information: Oxford University Press
Publication Year: 2019
Collection: Directory of Open Access Journals: DOAJ Articles
Subject Terms: Botany; QK1-989; Plant culture; SB1-1110
Description: A sweet solution for studying cacao genetics Genomic insights from an efficient, low-cost DNA sequencing strategy could enable more sustainable cacao cultivation. Growing demand for chocolate is currently being satisfied by increasing land use. A richer understanding of this plant’s genome could enable development of more productive strains, and Juan Carlos Motamayor and colleagues at Mars Chocolate have demonstrated a method for rapidly collecting such data. Most sequencing platforms produce vast multitudes of short DNA sequences which must then be assembled into a final map—a particular challenge for cacao, whose genome contains complex repetitive regions that are tough to reconstruct. Using a newer platform known as MinION, which produces far longer sequence reads, Motamayor’s team assembled a detailed genome map for a widely-used cacao cultivar within months for under $5,000. This approach could facilitate genetic engineering efforts to improve crop performance.
Document Type: article in journal/newspaper
Language: English
Relation: https://doi.org/10.1038/s41438-019-0125-7; https://doaj.org/toc/2052-7276; https://doaj.org/article/f475d725bad149459e407d58948066b1
DOI: 10.1038/s41438-019-0125-7
Availability: https://doi.org/10.1038/s41438-019-0125-7; https://doaj.org/article/f475d725bad149459e407d58948066b1
Accession Number: edsbas.C51D1836
Database: BASE