| Title: |
Potent synergy and sustained bactericidal activity of polymyxins combined with Gram-positive only class of antibiotics versus four Gram-negative bacteria |
| Authors: |
Wang, Yan; Feng, Jianwen; Yu, Jiameng; Wen, Lirong; Chen, Lidan; An, Huijie; Xiao, Weibin; Zhang, Bing; Feng, Huanhuan; Zhou, Mou; Jiang, Zhihui |
| Contributors: |
Natural Science Foundation of Guangdong Province Youth Enhancement Project; Key Basic Project of General Hospital of Southern Theatre Command; Science and Technology Program of Guangzhou |
| Source: |
Annals of Clinical Microbiology and Antimicrobials ; volume 23, issue 1 ; ISSN 1476-0711 |
| Publisher Information: |
Springer Science and Business Media LLC |
| Publication Year: |
2024 |
| Description: |
Background Gram-negative bacteria (GNB) are becoming increasingly resistant to a wide variety of antibiotics. There are currently limited treatments for GNB, and the combination of antibiotics with complementary mechanisms has been reported to be a feasible strategy for treating GNB infection. The inability to cross the GNB outer membrane (OM) is an important reason that a broad spectrum of Gram-positive only class of antibiotics (GPOAs) is lacking. Polymyxins may help GPOAs to permeate by disrupting OM of GNB. Objective To identify what kind of GPOAs can be aided to broaden their anti-GNB spectrum by polymyxins, we systematically investigated the synergy of eight GPOAs in combination with colistin (COL) and polymyxin B (PMB) against GNB in vitro. Methods The synergistic effect of COL or PMB and GPOAs combinations against GNB reference strains and clinical isolates were determined by checkerboard tests. The killing kinetics of the combinations were assessed using time-kill assays. Results In the checkerboard tests, polymyxins-GPOAs combinations exert synergistic effects characterized by species and strain specificity. The synergistic interactions on P. aeruginosa strains are significantly lower than those on strains of A. baumannii , K. pneumoniae and E. coli . Among all the combinations, COL has shown the best synergistic effect in combination with dalbavancin (DAL) or oritavancin (ORI) versus almost all of the strains tested, with FICIs from 0.16 to 0.50 and 0.13 to < 0.28, respectively. In addition, the time-kill assays demonstrated that COL/DAL and COL/ORI had sustained bactericidal activity. Conclusions Our results indicated that polymyxins could help GPOAs to permeate the OM of specific GNB, thus showed synergistic effects and bactericidal effects in the in vitro assays. In vivo combination studies should be further conducted to validate the results of this study. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| DOI: |
10.1186/s12941-024-00720-4 |
| DOI: |
10.1186/s12941-024-00720-4.pdf |
| DOI: |
10.1186/s12941-024-00720-4/fulltext.html |
| Availability: |
https://doi.org/10.1186/s12941-024-00720-4; https://link.springer.com/content/pdf/10.1186/s12941-024-00720-4.pdf; https://link.springer.com/article/10.1186/s12941-024-00720-4/fulltext.html |
| Rights: |
https://creativecommons.org/licenses/by/4.0 ; https://creativecommons.org/licenses/by/4.0 |
| Accession Number: |
edsbas.C5764AAA |
| Database: |
BASE |