| Title: |
Influence of PRE orientation and spacing on the output of the synthetic promoter. |
| Authors: |
Sandrine Pinheiro; Mariona Nadal-Ribelles; Carme Solé; Vincent Vincenzetti; Yves Dusserre; Francesc Posas; Serge Pelet |
| Publication Year: |
2025 |
| Collection: |
The University of Auckland: Figshare |
| Subject Terms: |
Microbiology; Cell Biology; Genetics; Molecular Biology; Immunology; Developmental Biology; Science Policy; Infectious Diseases; Plant Biology; Biological Sciences not elsewhere classified; two haploid cells; saccharomyces cerevisiae < p; core promoter modulate; ste12 binding sites; binding sites; strong dimer; specific timing; largely encoded; large diversity; basal association |
| Description: |
A, B and C. Time course of the nuclear enrichment of the dPSTR R for various distances of PRE placed in tail-to-head conformation towards the core (A), in tail-to-head conformation away from the core (B) and in head-to-head conformation (C). Three spacings are plotted in color. The solid lines represent the median and the shaded area the 25- to 75- percentile of the population. Gray lines represent the median of non-functional PRE conformations. The solid black line represents the median of the p SYN 3TT reference promoter. The black dashed line is the median of the control synthetic promoter without PRE. D, E and F. Summary graph displaying the expression output, the speed and the fraction of responding cells for various spacings of the two PREs placed in tail-to-head conformation towards the core (D), in tail-to-head conformation away from the core (E) and in head-to-head conformation (F). The color of the marker indicates the difference in response time between the synthetic promoter and the reference p AGA1 -dPSTR Y . The size of the marker represents the fraction of responding cells. The dashed line represents the expression output and the dashed dotted line the expression threshold calculated based on the p SYN 3TT . The O and T indicate a significant difference between the mean of the replicates (t-test: p-val < 0.05) in the timing of induction (T) or in the expression output (O) relative to the p SYN 3TT . (PDF) |
| Document Type: |
article in journal/newspaper |
| Language: |
unknown |
| Relation: |
https://figshare.com/articles/journal_contribution/Influence_of_PRE_orientation_and_spacing_on_the_output_of_the_synthetic_promoter_/29332255 |
| DOI: |
10.1371/journal.pgen.1011710.s004 |
| Availability: |
https://doi.org/10.1371/journal.pgen.1011710.s004; https://figshare.com/articles/journal_contribution/Influence_of_PRE_orientation_and_spacing_on_the_output_of_the_synthetic_promoter_/29332255 |
| Rights: |
CC BY 4.0 |
| Accession Number: |
edsbas.C5B726B8 |
| Database: |
BASE |