| Title: |
DataSheet1_Remodeling of the chromatin landscape in peripheral blood cells in patients with severe Delta COVID-19.pdf |
| Authors: |
Vasiliy E. Akimov; Dmitriy I. Tychinin; Olga A. Antonova; Abusaid M. Shaymardanov; Maria D. Voronina; Kseniia A. Deinichenko; Oleg D. Fateev; Vladimir S. Yudin; Sergey M. Yudin; Vladimir E. Mukhin; Svetlana V. Romanova; Aleksandra I. Nekrasova; Anastasia S. Zhdanova; Anastasia V. Tsypkina; Ivan S. Vladimirov; Antonida V. Makhotenko; Anton A. Keskinov; Sergey A. Kraevoy; Ekaterina A. Snigir; Dmitry V. Svetlichnyy; Veronika I. Skvortsova |
| Publication Year: |
2024 |
| Collection: |
Frontiers: Figshare |
| Subject Terms: |
Immunology; Applied Immunology (incl. Antibody Engineering; Xenotransplantation and T-cell Therapies); Autoimmunity; Cellular Immunology; Humoural Immunology and Immunochemistry; Immunogenetics (incl. Genetic Immunology); Innate Immunity; Transplantation Immunology; Tumour Immunology; Immunology not elsewhere classified; Genetic Immunology; Animal Immunology; Veterinary Immunology; scATAC-seq; single cell; COVID-19; transcriptional regulatory network; PBMC (peripheral blood mononuclear cells) |
| Description: |
COVID-19 is characterized by systemic pro-inflammatory shifts with the development of serious alterations in the functioning of the immune system. Investigations of the gene expression changes accompanying the infection state provide insight into the molecular and cellular processes depending on the sickness severity and virus variants. Severe Delta COVID-19 has been characterized by the appearance of a monocyte subset enriched for proinflammatory gene expression signatures and a shift in ligand–receptor interactions. We profiled the chromatin accessibility landscape of 140,000 nuclei in PBMC samples from healthy individuals or individuals with COVID-19. We investigated cis-regulatory elements and identified the core transcription factors governing gene expression in immune cells during COVID-19 infection. In severe cases, we discovered that regulome and chromatin co-accessibility modules were significantly altered across many cell types. Moreover, cases with the Delta variant were accompanied by a specific monocyte subtype discovered using scATAC-seq data. Our analysis showed that immune cells of individuals with severe Delta COVID-19 underwent significant remodeling of the chromatin accessibility landscape and development of the proinflammatory expression pattern. Using a gene regulatory network modeling approach, we investigated the core transcription factors governing the cell state and identified the most pronounced chromatin changes in CD14+ monocytes from individuals with severe Delta COVID-19. Together, our results provide novel insights into cis-regulatory module organization and its impact on gene activity in immune cells during SARS-CoV-2 infection. |
| Document Type: |
dataset |
| Language: |
unknown |
| DOI: |
10.3389/fimmu.2024.1415317.s001 |
| Availability: |
https://doi.org/10.3389/fimmu.2024.1415317.s001; https://figshare.com/articles/dataset/DataSheet1_Remodeling_of_the_chromatin_landscape_in_peripheral_blood_cells_in_patients_with_severe_Delta_COVID-19_pdf/27978030 |
| Rights: |
CC BY 4.0 |
| Accession Number: |
edsbas.C624D20D |
| Database: |
BASE |