| Title: |
Spatial proteomics finds CD155 and Endophilin-A1 as mediators of growth and invasion in medulloblastoma |
| Authors: |
Capdeville, Charles; Russo, Linda; Penton, David; Migliavacca, Jessica; Zecevic, Milica; Gries, Alexandre; Neuhauss, Stephan C F; Grotzer, Michael A; Baumgartner, Martin |
| Source: |
Capdeville, Charles; Russo, Linda; Penton, David; Migliavacca, Jessica; Zecevic, Milica; Gries, Alexandre; Neuhauss, Stephan C F; Grotzer, Michael A; Baumgartner, Martin (2022). Spatial proteomics finds CD155 and Endophilin-A1 as mediators of growth and invasion in medulloblastoma. Life Science Alliance, 5(6):e202201380. |
| Publisher Information: |
Life Science Alliance |
| Publication Year: |
2022 |
| Collection: |
University of Zurich (UZH): ZORA (Zurich Open Repository and Archive |
| Subject Terms: |
Medical Clinic; 570 Life sciences; biology; 610 Medicine & health |
| Description: |
The composition of the plasma membrane (PM)-associated proteome of tumor cells determines cell-cell and cell-matrix interactions and the response to environmental cues. Whether the PM-associated proteome impacts the phenotype of Medulloblastoma (MB) tumor cells and how it adapts in response to growth factor cues is poorly understood. Using a spatial proteomics approach, we observed that hepatocyte growth factor (HGF)-induced activation of the receptor tyrosine kinase c-MET in MB cells changes the abundance of transmembrane and membrane-associated proteins. The depletion of MAP4K4, a pro-migratory effector kinase downstream of c-MET, leads to a specific decrease of the adhesion and immunomodulatory receptor CD155 and of components of the fast-endophilin-mediated endocytosis (FEME) machinery in the PM-associated proteome of HGF-activated MB cells. The decreased surface expression of CD155 or of the fast-endophilin-mediated endocytosis effector endophilin-A1 reduces growth and invasiveness of MB tumor cells in the tissue context. These data thus describe a novel function of MAP4K4 in the control of the PM-associated proteome of tumor cells and identified two downstream effector mechanisms controlling proliferation and invasiveness of MB cells. |
| Document Type: |
article in journal/newspaper |
| File Description: |
application/pdf |
| Language: |
English |
| ISSN: |
2575-1077 |
| Relation: |
https://www.zora.uzh.ch/id/eprint/224574/7/e202201380.full.pdf; https://www.zora.uzh.ch/id/eprint/224574/1/Capdeville_et.al._Life_Sci_alliance_2022_zora.pdf; info:pmid/35296518; urn:issn:2575-1077 |
| DOI: |
10.26508/lsa.202201380 |
| Availability: |
https://www.zora.uzh.ch/id/eprint/224574/; https://www.zora.uzh.ch/id/eprint/224574/7/e202201380.full.pdf; https://www.zora.uzh.ch/id/eprint/224574/1/Capdeville_et.al._Life_Sci_alliance_2022_zora.pdf; https://doi.org/10.26508/lsa.202201380 |
| Rights: |
info:eu-repo/semantics/openAccess ; Creative Commons: Attribution 4.0 International (CC BY 4.0) ; http://creativecommons.org/licenses/by/4.0/ |
| Accession Number: |
edsbas.C70892B8 |
| Database: |
BASE |