Identification of Gnr1p, a negative regulator of G? signalling in Schizosaccharomyces pombe, and its complementation by human G? subunits
| Title: | Identification of Gnr1p, a negative regulator of G? signalling in Schizosaccharomyces pombe, and its complementation by human G? subunits |
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| Authors: | Alan Goddard; Graham Ladds; Rachel Forfar; John Davey |
| Publication Year: | 2012 |
| Subject Terms: | C700 - Molecular biology; biophysics & biochemistry; Gβ subunit; Gnr1p; Gpa1p; Pheromone; Schizosaccharomyces pombe; WD repeat protein |
| Description: | G protein-coupled receptors (GPCRs) are involved in the response of eukaryotic cells to a wide variety of stimuli, traditionally mediating their effects through heterotrimeric G proteins comprised of G?, G? and G? subunits. The fission yeast Schizosaccharomyces pombe is an established tool for GPCR research, possessing two G?-dependent signalling cascades. A complete G??? complex has been characterised for the glucose-sensing pathway, but only the G? subunit, Gpa1p, has been identified in the pheromone-response pathway. Here, we report the use of the yeast two-hybrid system to identify a novel protein, Gnr1p, which interacts with Gpa1p. Gnr1p is predicted to contain seven WD repeats and to adopt a structure similar to typical G? subunits. Disruption and overexpression studies reveal that Gnr1p negatively regulates the pheromone-response pathway but is not required for signalling. Human G? subunits complement the loss of Gnr1p, functioning as negative regulators of G? signalling in fission yeast. |
| Document Type: | article in journal/newspaper |
| Language: | unknown |
| Relation: | 10779/lincoln.24403597.v2; https://figshare.com/articles/journal_contribution/Identification_of_Gnr1p_a_negative_regulator_of_G_signalling_in_Schizosaccharomyces_pombe_and_its_complementation_by_human_G_subunits/24403597 |
| Availability: | https://figshare.com/articles/journal_contribution/Identification_of_Gnr1p_a_negative_regulator_of_G_signalling_in_Schizosaccharomyces_pombe_and_its_complementation_by_human_G_subunits/24403597 |
| Rights: | CC BY 4.0 |
| Accession Number: | edsbas.C7A9B0A5 |
| Database: | BASE |