| Title: |
O-148 Cumulative embryotoxic effect of IVF plastic devices used sequentially in routine clinical practice could slow the embryo development and delay blastulation |
| Authors: |
Delaroche, L; Besnard, L; Acacio, M; Mestres, E; Costa-Borges, N |
| Source: |
Human Reproduction ; volume 38, issue Supplement_1 ; ISSN 0268-1161 1460-2350 |
| Publisher Information: |
Oxford University Press (OUP) |
| Publication Year: |
2023 |
| Description: |
Study question Is there an embryotoxic cumulative effect resulting from IVF plastic consumables (PC) when used sequentially under clinical routine practice? Summary answer Reduced blastulation rates and slow development were detected in some associations of PC evaluated by the Mouse Embryo Assay (MEA) suggesting possible cumulative embryotoxicity. What is known already Recommendations from European (ESHRE) and American (ASRM) societies regarding the use of IVF PC suggest that they should undergo appropriate quality control tests to detect the possible presence of embryotoxins. The main test used by manufacturers to exclude any embryotoxicity is the MEA. This assay is currently performed individually on each PC so that a certificate of conformity is delivered for each product/lot. However, several PC are used during a single IVF cycle, potentially creating an embryotoxic cumulative effect. To our knowledge, the cumulative toxicity of several PC used sequentially on gametes and embryos has not been studied so far. Study design, size, duration The objective of this prospective study was to determine if there is cumulative embryotoxicity when several PC are used together under clinical routine conditions. Ten associations, each containing 13 to 31 PC, were designed, and assessed for embryotoxicity using a MEA methodology. The tested combinations replicated the different steps used in an IVF cycle, including sperm collection and selection, oocyte pick-up, fertilization, embryo culture, embryo transfer, vitrification and thawing and sperm freezing. Participants/materials, setting, methods A defined volume of culture media was used to extract the toxicity of several PC used in every association in triplicate. The MEA tests were performed on each medium extraction with 21 one-cell stage fresh mouse embryos. Blastocyst formation rates after 96 and 120h of culture (Day 5/6), blastocyst good quality rates and hatched blastocyst rates were compared between the tested and control groups. The total cell number per ... |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| DOI: |
10.1093/humrep/dead093.175 |
| Availability: |
https://doi.org/10.1093/humrep/dead093.175; https://academic.oup.com/humrep/article-pdf/38/Supplement_1/dead093.175/50787336/dead093.175.pdf |
| Rights: |
https://academic.oup.com/pages/standard-publication-reuse-rights |
| Accession Number: |
edsbas.C7C9FCD2 |
| Database: |
BASE |