| Title: |
www.mdpi.com/journal/ijerph Elevated Oestrogen Receptor Splice Variant ER��5 Expression in Tumour-adjacent Hormone-responsive Tissue |
| Authors: |
Siân E. Taylor; Imran I. Patel; Paras B. Singh; Caroline M. Nicholson; Helen F. Stringfellow; R. K. Gopala Krishna; Shyam S. Matanhelia; Pierre L. Martin-hirsch; Francis L. Martin |
| Contributors: |
The Pennsylvania State University CiteSeerX Archives |
| Source: |
ftp://ftp.ncbi.nlm.nih.gov/pub/pmc/91/35/Int_J_Environ_Res_Public_Health_2010_Nov_2_7(11)_3871-3889.tar.gz |
| Publication Year: |
2010 |
| Collection: |
CiteSeerX |
| Description: |
Susceptibility to prostate or endometrial cancer is linked with obesity, a state of oestrogen excess. Oestrogen receptor (ER) splice variants may be responsible for the tissue-level of ER activity. Such micro-environmental regulation may modulate cancer initiation and/or progression mechanisms. Real-time reverse transcriptase (RT) polymerase chain reaction (PCR) was used to quantitatively assess the levels of four ER splice variants (ER��3, ER��5, ER�2 and ER�5), plus the full-length parent isoforms ER � and ER�1, in high-risk [tumour-adjacent prostate (n = 10) or endometrial cancer (n = 9)] vs. low-risk [benign prostate (n = 12) or endometrium (n = 9)], as well as a comparison of UK (n = 12) vs. Indian (n = 15) benign prostate. All three tissue groups expressed the ER splice variants at similar levels, apart from ER��5. This splice variant was markedly raised in all of the tumour-adjacent prostate samples compared to benign tissues. Immunofluorescence analysis for ER�2 in prostate tissue demonstrated that such splice variants are present inInt. J. Environ. Res. Public Health 2010, 7 3872 |
| Document Type: |
text |
| File Description: |
application/zip |
| Language: |
English |
| Relation: |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.292.6173 |
| Availability: |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.292.6173 |
| Rights: |
Metadata may be used without restrictions as long as the oai identifier remains attached to it. |
| Accession Number: |
edsbas.C7D0F9D5 |
| Database: |
BASE |