| Title: |
Ecotoxicology and phylogeography studies of the cockle Cerastoderma glaucum from the gulf of Gabès : adaptive response (in situ and in vivo) to metal stress and genetic structure ; Etude écotoxicologique et phylogéographique de la coque Cerastoderma glaucum issue du Golfe de Gabès : réponse adaptative (in situ et in vivo) au stress métallique et structure génétique |
| Authors: |
Karray, Sahar |
| Contributors: |
Mer, molécules et santé EA 2160 (MMS); Le Mans Université (UM)-Université de Nantes - UFR des Sciences et des Techniques (UN UFR ST); Université de Nantes (UN)-Université de Nantes (UN)-Université de Nantes - UFR des Sciences Pharmaceutiques et Biologiques (UFR SPB); Université de Nantes (UN)-Université de Nantes (UN); Le Mans Université; Université de Sfax (Tunisie); Benoît Chénais; Amel Hamza-Chaffai |
| Source: |
https://theses.hal.science/tel-01260002 ; Ecotoxicologie. Le Mans Université; Université de Sfax (Tunisie), 2015. Français. ⟨NNT : 2015LEMA1007⟩. |
| Publisher Information: |
CCSD |
| Publication Year: |
2015 |
| Collection: |
Université de Nantes: HAL-UNIV-NANTES |
| Subject Terms: |
Phylogéography; Controlled conditions; Natural conditions; Biomarkers; Western Mediterranean sea; Ecotoxicology; Conditions contrôlées; Milieu naturel; Biomarqueurs; Méditerranée occidentale; Golfe de Gabès; Phylogéographie; Ecotoxicologie; Cerastoderma glaucum; [SDV.TOX.ECO]Life Sciences [q-bio]/Toxicology/Ecotoxicology |
| Description: |
Cerastoderma glaucum is an adequate biological model for phylogeographic and ecotoxicological studies.A study of its genetic structure in the Western Mediterranean sea was conducted using two markers types: mitochondrial and nuclear. The DNA mitochondrial marker results show a divergent population from all the other ones (Ellouza South ofTunisia).An ecotoxicological study of populations sampled from Ellouza site and two other nearby sites in the Gulf of Gabes region was carried out in order to study the adaptive response of natural populations of C. glaucum at different levels of biological organization from the individual level to the cellular and molecular ones. In parallel, two laboratory experiments were performed to clarify the mechanisms that may explain the success or failure of maintaining populations of C. glaucum. In thefirst one, industrial effluents were used and in the second we have used a pure contaminant, the cadmium. These studies experiments concerned individual and molecular levels. Different biomarkers involved in the response to different types of stress: (MT), (ABCB1) (MnSOD and CuZnSOD) (CAT) (HSP70) and (COI) were used in natural and exposed cockles.Results showed the importance of energy metabolism in response to stress whatever the used approach (in situ / Experimental) through the regulation of the expression of the COI gene. ; Cerastoderma glaucum est un modèle biologique de choix pour des études phylogéographiques et écotoxicologiques.Une étude de sa structure génétique en méditerranée occidentale a été réalisée en utilisant deux types de marqueurs, mitochondrial et nucléaire. Les résultats obtenus ont révélé à travers l’ADN mitochondrial une population très divergente de toutes les autres populations (Ellouza, Sud de la Tunisie).Une étude écotoxicologique sur des populations issues de ce site Ellouza et deux autres sites à proximité dans la région du golfe de Gabès a été réalisée dans le but d’étudier la réponse adaptative des populations de la coque C. glaucum en milieu naturel à ... |
| Document Type: |
doctoral or postdoctoral thesis |
| Language: |
French |
| Relation: |
NNT: 2015LEMA1007 |
| Availability: |
https://theses.hal.science/tel-01260002; https://theses.hal.science/tel-01260002v1/document; https://theses.hal.science/tel-01260002v1/file/2015LEMA1007.pdf |
| Rights: |
https://about.hal.science/hal-authorisation-v1/ ; info:eu-repo/semantics/OpenAccess |
| Accession Number: |
edsbas.C844D712 |
| Database: |
BASE |