| Title: |
Endocytosis of Activated Muscarinic m2 Receptor (m2R) in Live Mouse Hippocampal Neurons Occurs via a Clathrin-Dependent Pathway |
| Authors: |
Lambert, Lisa; Dubayle, David; Fafouri, Assia; Herzog, Etienne; Csaba, Zsolt; Dournaud, Pascal; El Mestikawy, Salah; Bernard, Véronique |
| Contributors: |
Neuroscience Paris Seine (NPS); Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut de Biologie Paris Seine (IBPS); Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS); Institut de Biologie Paris Seine (IBPS); Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS); Sorbonne Université (SU); Sorbonne Université - Faculté de Médecine (SU FM); Systèmes glutamatergiques normaux et pathologiques = Normal and Pathologic Glutamatergic Neurons (NPS); Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut de Biologie Paris Seine (IBPS); Centre de neurophysique, physiologie, pathologie (UMR 8119); Université Paris Descartes - Paris 5 (UPD5)-Centre National de la Recherche Scientifique (CNRS); Neuroprotection du Cerveau en Développement / Promoting Research Oriented Towards Early Cns Therapies (PROTECT); Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Robert Debré; Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Université Paris Diderot - Paris 7 (UPD7)-Institut National de la Santé et de la Recherche Médicale (INSERM); Interdisciplinary Institute for Neuroscience / Institut interdisciplinaire de neurosciences Bordeaux (IINS); Université de Bordeaux (UB)-Centre National de la Recherche Scientifique (CNRS); McGill University = Université McGill Montréal, Canada; ANR-09-MNPS-0033,PSYVGLUT,VGLUT3 et maladies psychiatriques(2009); ANR-13-SAMA-0005,COCACh,VGLUT3, les interneurones cholinergiques striataux et l'addiction(2013) |
| Source: |
ISSN: 1662-5102 ; Frontiers in Cellular Neuroscience ; https://hal.sorbonne-universite.fr/hal-01977271 ; Frontiers in Cellular Neuroscience, 2018, 12, pp.450. ⟨10.3389/fncel.2018.00450⟩. |
| Publisher Information: |
CCSD; Frontiers |
| Publication Year: |
2018 |
| Subject Terms: |
trafficking; time lapse confocal microscopy; mouse; internalization; G protein-coupled receptor; [SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology |
| Description: |
International audience ; Our aim was to examine the dynamics of the muscarinic m2 receptor (m2R), a G-protein coupled receptor (GPCR), after agonist activation in living hippocampal neurons, and especially clathrin dependency endocytosis. We have previously shown that the m2R undergoes agonist-induced internalization in vivo. However, the nature of the endocytotic pathway used by m2R after activation is still unknown in living neurons. Using live cell imaging and quantitative analyses, we have monitored the effect of stimulation on the fate of the membrane-bound m2R and on its redistribution in intraneuronal compartments. Shortly (6 min) after activation, m2R is internalized into clathrin immunopositive structures. Furthermore, after clathrin-dependent endocytosis, m2R associates with early and late endosomes and with subcellular organelles involved in degradation. Together, these results provide, for the first time, a description of m2R trafficking in living neurons and prove that m2R undergoes clathrin-dependent endocytosis before being degraded. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| Relation: |
info:eu-repo/semantics/altIdentifier/pmid/30555302; PUBMED: 30555302; PUBMEDCENTRAL: PMC6283979 |
| DOI: |
10.3389/fncel.2018.00450 |
| Availability: |
https://hal.sorbonne-universite.fr/hal-01977271; https://hal.sorbonne-universite.fr/hal-01977271v1/document; https://hal.sorbonne-universite.fr/hal-01977271v1/file/Lambert%20et%20al%20051118.pdf; https://doi.org/10.3389/fncel.2018.00450 |
| Rights: |
info:eu-repo/semantics/OpenAccess |
| Accession Number: |
edsbas.CA1DA423 |
| Database: |
BASE |