| Title: |
Ghrelin/motilin-related peptide is a potent prokinetic to reverse gastric postoperative ileus in rat |
| Authors: |
Trudel, Louise; Tomasetto, Catherine; Rio, Marie-Christine; Bouin, Mickaël; Plourde, Victor; Eberling, Pascal; Poitras, Pierre |
| Contributors: |
Institut de génétique et biologie moléculaire et cellulaire (IGBMC); Université Louis Pasteur - Strasbourg I-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS); Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC); Université de Strasbourg (UNISTRA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS) |
| Source: |
ISSN: 0193-1857. |
| Publisher Information: |
HAL CCSD; American Physiological Society |
| Publication Year: |
2002 |
| Collection: |
Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
| Subject Terms: |
[SDV.MHEP]Life Sciences [q-bio]/Human health and pathology |
| Description: |
A novel peptide called ghrelin or motilin-related-peptide (MTLRP) was found in the stomach of various mammals. We studied its effect on the motor function of the rat gastrointestinal tract. In normal, conscious unoperated animals, ghrelin/MTLRP (5 or 20 microg/kg iv) significantly accelerated the gastric emptying of a methylcellulose liquid solution (gastric residue after 15 min: 57 +/- 7, 42 +/- 11, 17 +/- 4, and 9 +/- 3% of the ingested meal with doses of 0, 1, 5, and 20 microg/kg iv, respectively) Transit of the methylcellulose liquid solution was also accelerated by ghrelin/MTLRP in the small intestine but not in the colon. Des-[Gln(14)]ghrelin, also found in the mammalian stomach, was as potent as ghrelin in emptying the stomach (gastric residue after 15 min: 12 +/- 3% at a dose of 20 microg/kg iv). In rats in which postoperative gastrointestinal ileus had been experimentally induced, ghrelin/MTLRP (20 microg/kg iv) reversed the delayed gastric evacuation (gastric residue after 15 min: 28 +/- 7% of the ingested meal vs. 82 +/- 9% with saline). In comparison, the gastric ileus was not modified by high doses of motilin (77 +/- 7%) or erythromycin (82 +/- 6%) and was only partially improved by calcitonin gene-related peptide (CGRP) 8-37 antagonist (59 +/- 7%). Ghrelin/MTLRP, therefore, accelerates the gastric emptying and small intestinal transit of a liquid meal and is a strong prokinetic agent capable of reversing the postoperative gastric ileus in rat. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| Relation: |
hal-04146331; https://hal.science/hal-04146331 |
| DOI: |
10.1152/ajpgi.00339.2001 |
| Availability: |
https://hal.science/hal-04146331; https://doi.org/10.1152/ajpgi.00339.2001 |
| Accession Number: |
edsbas.CAAB2A44 |
| Database: |
BASE |