| Title: |
A SHERLOCK toolbox for the eco-epidemiological surveillance of animal African trypanosomosis reveals a similar parasite diversity in domestic pigs in two ancient sleeping sickness foci in Western Africa |
| Authors: |
Eloiflin, Roger-Junior; Pérez-Antón, Elena; Camara, Aïssata; Dujeancourt-Henry, Annick; Boiro, Salimatou; N’djetchi, Martial; Taoré, Mélika; Koffi, Mathurin; Kaba, Dramane; Le Pennec, Yann; Doukouré, Bakary; Camara, Abdoulaye Dansy; Kagbadouno, Moïse; Campagne, Pascal; Camara, Mamadou; Jamonneau, Vincent; Thevenon, Sophie; Bart, Jean-Mathieu; Glover, Lucy; Rotureau, Brice |
| Contributors: |
Institut de recherche pour le développement IRD : UMR237; Biologie moléculaire des Trypanosomes - Trypanosome Molecular Biology; Institut Pasteur Paris (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPCité); Institut Pasteur de Guinée; Pasteur Network (Réseau International des Instituts Pasteur); Université Jean Lorougnon Guédé (UJloG); Institut Pierre Richet (IPR); Ministère de la Santé Conakry, Guinea; Hub Bioinformatique et Biostatistique - Bioinformatics and Biostatistics HUB; Institut Pasteur Paris (IP)-Université Paris Cité (UPCité); Interactions hôtes-vecteurs-parasites-environnement dans les maladies tropicales négligées dues aux trypanosomatides (UMR INTERTRYP); Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut de Recherche pour le Développement (IRD)-Université de Montpellier (UM); Biologie cellulaire des Trypanosomes - Trypanosome Cell Biology; This project was funded by the Agence Nationale pour la Recherche (ANR-21-CE17-0022-01 SherPa), the Programme Investissement d’Avenir of the French Government through the two Laboratoires d’Excellence, ANR-10-LABX-62-IBEID and ANR-11-LABX-0024-PARAFRAP, and the Pasteur Network (Bourse Calmette-Yersin to AC).; ANR-21-CE17-0022,SherPa,Développement d'une plateforme SHERLOCK pour la détection polyvalente des parasites trypanosomatidés.(2021); ANR-10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases(2010); ANR-11-LABX-0024,ParaFrap,Alliance française contre les maladies parasitaires(2011) |
| Source: |
https://hal.science/hal-05130117 ; 2025. |
| Publisher Information: |
CCSD |
| Publication Year: |
2025 |
| Subject Terms: |
[SDV]Life Sciences [q-bio]; [SDV.MP.PAR]Life Sciences [q-bio]/Microbiology and Parasitology/Parasitology |
| Description: |
International audience ; Animal African trypanosomosis (AAT), caused by protist parasites of the genus Trypanosoma, puts upward of a million head of livestock at risk across 37 countries in Africa. The economic impact of AAT and the presence of human-infectious trypanosomes in animals place a clear importance on improving diagnostics for animal trypanosomes to map the distribution of the veterinary parasites and identify reservoirs of human-infectious trypanosomes. We have adapted the CRISPR-based detection toolkit SHERLOCK (Specific High-sensitivity Enzymatic Reporter unLOCKing) for trypanosomatid parasites responsible for AAT (SHERLOCK4AAT) including Pan-trypanosomatid, Trypanozoon, T. vivax, T. congolense, T. theileri, T. simiae and T. suis assays. To test the applicability of this technique in the field, we analysed dried blood spots collected from 200 farm and 224 free-ranging pigs in endemic and historical human African trypanosomiasis foci in Guinea and Côte d’Ivoire, respectively. The results revealed that SHERLOCK4AAT can detect and discriminate between trypanosome species involved in multiple infections with a high sensitivity. 62.7 % [58.1, 67.3] of pigs were found infected with at least one trypanosome species. T. brucei gambiense, a human-infectious trypanosome, was found in one animal at both sites, highlighting the risk that these animals may act as persistent reservoirs. These data suggest that, due to their proximity to humans and their attractiveness to tsetse flies, pigs could act as sentinels to monitor T. b. gambiense circulation using the SHERLOCK4AAT toolbox. |
| Document Type: |
report |
| Language: |
English |
| DOI: |
10.7554/eLife.106823.1 |
| Availability: |
https://hal.science/hal-05130117; https://hal.science/hal-05130117v1/document; https://hal.science/hal-05130117v1/file/106823-v1.pdf; https://doi.org/10.7554/eLife.106823.1 |
| Rights: |
http://creativecommons.org/licenses/by/ ; info:eu-repo/semantics/OpenAccess |
| Accession Number: |
edsbas.CB18A38F |
| Database: |
BASE |