| Title: |
Triggered Drug Release from Dynamic Microspheres via a Protein Conformational Change |
| Authors: |
King, William J.; Pytel, Nicholas J.; Ng, Kelvin; Murphy, William L. |
| Contributors: |
NSF-funded |
| Source: |
Macromolecular Bioscience ; volume 10, issue 6, page 580-584 ; ISSN 1616-5187 1616-5195 |
| Publisher Information: |
Wiley |
| Publication Year: |
2010 |
| Collection: |
Wiley Online Library (Open Access Articles via Crossref) |
| Description: |
In this study we formed and characterized dynamic hydrogel microspheres in which a protein conformational change was used to control microsphere volume changes and the release of an encapsulated drug. In particular, a specific biochemical ligand, trifluoperazine, induced calmodulin's nanometer scale conformation change, which translated to a 48.7% microsphere volume decrease. This specific, ligand‐induced volume change triggered the release of a model drug, vascular endothelial growth factor (VEGF), at pre‐determined times. After release from the microspheres, 85.6 ± 10.5% of VEGF was in its native conformation. Taken together, these results suggest that protein conformational change could serve as a useful mechanism to control drug release from dynamic hydrogels. magnified image |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| DOI: |
10.1002/mabi.200900382 |
| Availability: |
https://doi.org/10.1002/mabi.200900382; https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fmabi.200900382; https://onlinelibrary.wiley.com/doi/pdf/10.1002/mabi.200900382 |
| Rights: |
http://onlinelibrary.wiley.com/termsAndConditions#vor ; http://doi.wiley.com/10.1002/tdm_license_1.1 ; http://onlinelibrary.wiley.com/termsAndConditions#vor |
| Accession Number: |
edsbas.CB75816D |
| Database: |
BASE |