| Title: |
A Western-Style Breakfast Induces a More Pro-Inflammatory Postprandial Response and Promotes Greater Macrophage Lipid Accumulation Compared to a Mediterranean-Style Breakfast in Obese and Normal-Weight Individuals |
| Authors: |
Alejandro Matamoros-Domínguez; Laura Sinausia; Gisela Pérez-Muñoz; Juan Manuel Espinosa-Cabello; Aída García-González; Ana Rodríguez-Rodríguez; José María Castellano; Elena María Yubero-Serrano; Emilio Montero; Javier S. Perona |
| Source: |
Nutrients ; Volume 18 ; Issue 4 ; Pages: 672 |
| Publisher Information: |
Multidisciplinary Digital Publishing Institute |
| Publication Year: |
2026 |
| Collection: |
MDPI Open Access Publishing |
| Subject Terms: |
postprandial metabolism; triglyceride-rich lipoproteins; dietary fat; inflammation; obesity |
| Subject Geographic: |
agris |
| Description: |
Background and objectives: Since postprandial lipid metabolism has emerged as a risk factor for cardiovascular disease, the quality of dietary fat may have a crucial role in atherogenesis and metabolic inflammation. In this study, we propose that the quality of dietary fats and the metabolic status of individuals modulate postprandial triglyceride-rich lipoprotein (TRL) composition and the response of macrophages to TRL. Methods: Randomized controlled crossover trial in the postprandial phase in 12 normal-weight adults and 12 adults with obesity. Each participant consumed both a Western-style (WB) and a Mediterranean-style (MB) breakfast in separate sessions, containing butter or olive oil as the fat source, respectively. Blood samples were collected at baseline (0 h), 2 h, and 4 h postprandially, and TRL were isolated and used to treat THP-1 macrophages. Results: The intake of the WB led to higher concentrations of inflammatory-related markers, particularly in individuals with obesity, and resulted in a higher content of saturated fatty acids and lower of monounsaturated fatty acids in TRL compared to the MB. Staining TRL-treated macrophages with Oil Red O revealed substantial lipid accumulation, which was more pronounced in cells cultured with 4 h TRL from individuals with obesity. This was also evidenced by upregulation of gene expression of lipoprotein uptake receptors following the consumption of the WB. Conclusions: Consumption of a WB led to a more pro-inflammatory postprandial profile and promoted greater lipid accumulation in macrophages, particularly in individuals with obesity, compared to a MB. These findings highlight the importance of fat quality in meals for cardiovascular risk management, especially in populations with obesity. |
| Document Type: |
text |
| File Description: |
application/pdf |
| Language: |
English |
| Relation: |
Lipids; https://dx.doi.org/10.3390/nu18040672 |
| DOI: |
10.3390/nu18040672 |
| Availability: |
https://doi.org/10.3390/nu18040672 |
| Rights: |
https://creativecommons.org/licenses/by/4.0/ |
| Accession Number: |
edsbas.CBB69C02 |
| Database: |
BASE |