| Title: |
Molecular Characterization of Gloecapsomorpha Prisca Microfossils by Mass Spectrometry Down to the Cellular Scale |
| Authors: |
Bon, Mathilde; Lepot, Kévin; Carpentier, Yvain; Bray, Fabrice; Riboulleau, Armelle; Baudin, François; Nuns, Nicolas; Rolando, Christian; Steemans, Philippe; Vandenbroucke, Thijs R. A. |
| Contributors: |
Laboratoire d’Océanologie et de Géosciences (LOG) - UMR 8187 (LOG); Institut national des sciences de l'Univers (INSU - CNRS)-Université du Littoral Côte d'Opale (ULCO)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD Ile-de-France ); Laboratoire de Physique des Lasers, Atomes et Molécules - UMR 8523 (PhLAM); Université de Lille-Centre National de la Recherche Scientifique (CNRS); Universiteit Gent = Ghent University = Université de Gand (UGent); Institut universitaire de France (IUF); Ministère de l'Education nationale, de l’Enseignement supérieur et de la Recherche (M.E.N.E.S.R.); Physique Moléculaire aux Interfaces (PMI); Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université de Lille-Centre National de la Recherche Scientifique (CNRS); Miniaturisation pour la Synthèse, l’Analyse et la Protéomique - UAR 3290 (MSAP); Institut de Chimie - CNRS Chimie (INC-CNRS)-Université de Lille-Centre National de la Recherche Scientifique (CNRS); Institut des Sciences de la Terre de Paris (iSTeP); Institut national des sciences de l'Univers (INSU - CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS); Unité de Catalyse et Chimie du Solide - UMR 8181 (UCCS); Université d'Artois (UA)-Centrale Lille-Institut de Chimie - CNRS Chimie (INC-CNRS)-Université de Lille-Centre National de la Recherche Scientifique (CNRS); Paléobotanique, Paléopalynologie, Micropaléontologie (Liège, Belgium); Université de Liège = University of Liège = Universiteit van Luik = Universität Lüttich (ULiège); ANR-22-PEXF-0001,Pilotage et gouvernance du programme FairCarboN,Pilotage et gouvernance du programme FairCarboN(2022) |
| Source: |
31st International Meeting on Organic Geochemistry (IMOG 2023); https://hal.science/hal-04525522; 31st International Meeting on Organic Geochemistry (IMOG 2023), Sep 2023, Montpellier, France. pp.1-2, ⟨10.3997/2214-4609.202333163⟩; https://imogconference.org/imog-23/ |
| Publisher Information: |
CCSD; European Association of Geoscientists & Engineers |
| Publication Year: |
2023 |
| Collection: |
LillOA (HAL Lille Open Archive, Université de Lille) |
| Subject Terms: |
mass spectrometry; micropaleontology; geobiochemistry; acritarchs; kukersite; [SDE]Environmental Sciences; [CHIM]Chemical Sciences |
| Subject Geographic: |
Montpellier; France |
| Description: |
International audience ; Gloeocapsomorpha prisca (Fig. 1) is a microfossil (∼10µm) with an organic wall of uncertain biological affinity, probably a cyanobacterium1,2,4,6. This fossil microorganism constitutes the vast majority of the organic matter in “kukersite” rocks. G. prisca has been thoroughly studied by gas chromatography-mass spectrometry (GC-MS), using extractive techniques, chemolysis and pyrolysis1,2,3. Here we perform a pilot study in order to investigate the potential of laser-assisted mass spectrometry to analyze the molecular fingerprint of single microfossils. Bitumen and kerogen of a kukersite sample from a 460-million-year-old deposit of northwestern Russia5, were analyzed after organic solvent extractions and demineralization. We measure bulk thermo-desorption and pyrolysis parameters with Rock-Eval 7S. We use molecular characterization methods that allow analyses down to the scale of a single microfossil. We analyze kerogen by secondary ion time-of-flight mass spectrometry (ToF-SIMS), which combines extreme surface sensitivity with a spatial resolution of ∼1 µm. The kerogen is then analyzed with high resolution (HR-µL2-MS) two-step laser desorption-ionization mass spectrometry with a spatial resolution of 140 µm. We used very-high mass resolution Fourier transform ion cyclotron resonance mass spectrometry by laser desorption/ionization (LDI-FT-ICR-MS) on G. prisca aggregates (e.g., Fig. 1) with laser beams down to less than 20 µm. |
| Document Type: |
conference object |
| Language: |
English |
| DOI: |
10.3997/2214-4609.202333163 |
| Availability: |
https://hal.science/hal-04525522; https://hal.science/hal-04525522v1/document; https://hal.science/hal-04525522v1/file/IMOG%202023.pdf; https://doi.org/10.3997/2214-4609.202333163 |
| Rights: |
https://about.hal.science/hal-authorisation-v1/ ; info:eu-repo/semantics/OpenAccess |
| Accession Number: |
edsbas.CC0DAFDF |
| Database: |
BASE |