| Title: |
Taurine Transporter Gene Expression in Mononuclear Blood Cells of Type 1 Diabetes Patients |
| Authors: |
Napoli, Zaleida; Seghieri, Giuseppe; Bianchi, Loria; Anichini, Roberto; De Bellis, Alessandra; Campesi, Ilaria; Carru, Ciriaco; Occhioni, Stefano; Zinellu, Angelo; Franconi, Flavia |
| Contributors: |
Fondazione Cassa di Risparmio di Pistoia e Pescia; Accademia Medica Pistoiese |
| Source: |
Journal of Diabetes Research ; volume 2016, page 1-7 ; ISSN 2314-6745 2314-6753 |
| Publisher Information: |
Wiley |
| Publication Year: |
2016 |
| Collection: |
Wiley Online Library (Open Access Articles via Crossref) |
| Description: |
Background . Taurine transporter gene expression (RNA-TauT) has a role in retinal cell function and is modulated in vitro and in vivo by hyperglycemia and/or oxidative stress. This study was aimed at testing whether RNA-TauT gene expression is modified in blood mononuclear peripheral cells (MPCs) of type 1 diabetic patients, is related to plasma markers of oxidative stress or endothelial dysfunction, or, finally, is related to presence of retinopathy. Methods . RNA-TauT was measured in MPCs by real-time PCR-analysis in 35 type 1 diabetic patients and in 33 age- and sex-matched controls, additionally measuring plasma and cell taurine and markers of oxidative stress and endothelial dysfunction. Results . RNA-TauT, expressed as 2 - Δ Δ C t , was significantly higher in MPCs of type 1 diabetic patients than in controls [median (interquartile range): 1.32(0.31) versus 1.00(0.15); P = 0.01 ]. In diabetic patients RNA-TauT was related to HbA1c ( r = 0.42 ; P = 0.01 ) and inversely to plasma homocysteine ( r = - 0.39 ; P = 0.02 ) being additionally significantly higher in MPCs of patients without retinopathy [( n = 22 ); 1.36(0.34)] compared to those with retinopathy [( n = 13 ); 1.16(0.20)], independently from HbA1c or diabetes duration. Conclusions . RNA-TauT gene expression is significantly upregulated in MPCs of type 1 diabetes patients and is related to HbA1c levels and inversely to plasma homocysteine. Finally, in diabetes patients, RNA-TauT upregulation seems to be blunted in patients with retinopathy independently of their metabolic control or longer diabetes duration. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| DOI: |
10.1155/2016/7313162 |
| Availability: |
https://doi.org/10.1155/2016/7313162; http://downloads.hindawi.com/journals/jdr/2016/7313162.pdf; http://downloads.hindawi.com/journals/jdr/2016/7313162.xml |
| Rights: |
http://creativecommons.org/licenses/by/4.0/ |
| Accession Number: |
edsbas.CC2F3780 |
| Database: |
BASE |