| Title: |
Syncrip/hnRNP Q is required for activity-induced Msp300/Nesprin-1 expression and new synapse formation |
| Authors: |
Titlow, J; Robertson, F; Järvelin, A; Ish-Horowicz, D; Smith, C; Gratton, E; Davis, I |
| Source: |
Journal of Cell Biology , 219 (3) , Article e201903135. (2020) |
| Publication Year: |
2020 |
| Collection: |
University College London: UCL Discovery |
| Subject Terms: |
Neuroscience; Physiology; RNA biology |
| Description: |
Memory and learning involve activity-driven expression of proteins and cytoskeletal reorganization at new synapses, requiring posttranscriptional regulation of localized mRNA a long distance from corresponding nuclei. A key factor expressed early in synapse formation is Msp300/Nesprin-1, which organizes actin filaments around the new synapse. How Msp300 expression is regulated during synaptic plasticity is poorly understood. Here, we show that activity-dependent accumulation of Msp300 in the postsynaptic compartment of the Drosophila larval neuromuscular junction is regulated by the conserved RNA binding protein Syncrip/hnRNP Q. Syncrip (Syp) binds to msp300 transcripts and is essential for plasticity. Single-molecule imaging shows that msp300 is associated with Syp in vivo and forms ribosome-rich granules that contain the translation factor eIF4E. Elevated neural activity alters the dynamics of Syp and the number of msp300:Syp:eIF4E RNP granules at the synapse, suggesting that these particles facilitate translation. These results introduce Syp as an important early acting activity-dependent regulator of a plasticity gene that is strongly associated with human ataxias. |
| Document Type: |
article in journal/newspaper |
| File Description: |
text |
| Language: |
English |
| Relation: |
https://discovery.ucl.ac.uk/id/eprint/10091238/1/Titilow%20et%20al%20JCB%20published%20Feb20.pdf; https://discovery.ucl.ac.uk/id/eprint/10091238/ |
| Availability: |
https://discovery.ucl.ac.uk/id/eprint/10091238/1/Titilow%20et%20al%20JCB%20published%20Feb20.pdf; https://discovery.ucl.ac.uk/id/eprint/10091238/ |
| Rights: |
open |
| Accession Number: |
edsbas.CD03BD20 |
| Database: |
BASE |