| Title: |
Nutrient restriction and hypoxia independently decrease placental cell proliferation and differentially regulate Igf2, Mtd and Phlda 2 gene expression in vitro |
| Authors: |
Brannon, Patsy M; Jones, Sara |
| Source: |
The FASEB Journal ; volume 24, issue S1 ; ISSN 0892-6638 1530-6860 |
| Publisher Information: |
Wiley |
| Publication Year: |
2010 |
| Collection: |
Wiley Online Library (Open Access Articles via Crossref) |
| Description: |
Maternal undernutrition impairs placental and fetal growth resulting in IUGR and developmental programming, but its mechanism and interaction with hypoxia are unknown. Our objectives were to determine the independent and interactive regulation of placental growth by undernutrition and hypoxia and establish a cell culture model of maternal undernutrition. HTR‐8/SVneo human trophoblast cells were cultured in RPMI media and 1.25%–5% FBS with 1–20% oxygen. 75% reduction (NR) of media glucose, essential amino acids, Gln, and vitamins maximally reduced growth. 1% oxygen (Hx) maximally decreased growth and increased Glut3 mRNA 7 fold, whereas 20% oxygen (Nx) maximized growth and minimized Glut 3 mRNA. In a 2×2 factorial experiment at 72 h, both NR and Hx independently reduced growth and Ki67 mRNA levels (p |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| DOI: |
10.1096/fasebj.24.1_supplement.544.2 |
| Availability: |
http://dx.doi.org/10.1096/fasebj.24.1_supplement.544.2 |
| Rights: |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
| Accession Number: |
edsbas.CE6C86F1 |
| Database: |
BASE |