| Title: |
Single-cell transcriptional diversity is a hallmark of developmental potential |
| Authors: |
Gulati, Gunsagar S.; Sikandar, Shaheen S.; Wesche, Daniel J.; Manjunath, Anoop; Bharadwaj, Anjan; Berger, Mark J.; Ilagan, Francisco; Kuo, Angera H.; Hsieh, Robert W.; Cai, Shang; Zabala, Maider; Scheeren, Ferenc A.; Lobo, Neethan A.; Qian, Dalong; Yu, Feiqiao B.; Dirbas, Frederick M.; Clarke, Michael F.; Newman, Aaron M. |
| Contributors: |
National Science Foundation; U.S. Department of Defense; National Cancer Institute; Stanford Bio-X Bowes Graduate Student Fellowship; Stanford Medical Science Training Program; Virginia and D.K. Ludwig Fund for Cancer Research; Stinehart-Reed foundation; Stanford Bio-X Interdisciplinary Initiatives Seed Grants Program; Breast Cancer Research Foundation |
| Source: |
Science ; volume 367, issue 6476, page 405-411 ; ISSN 0036-8075 1095-9203 |
| Publisher Information: |
American Association for the Advancement of Science (AAAS) |
| Publication Year: |
2020 |
| Description: |
More diversity at the top A detailed knowledge of cell differentiation hierarchies is important for understanding diverse biological processes such as organ development, tissue regeneration, and cancer. Single-cell RNA sequencing can help elucidate these hierarchies, but it requires reliable computational methods for predicting cell lineage trajectories. Gulati et al. developed CytoTRACE, a computational framework based on the simple observation that transcriptional diversity—the number of genes expressed in a cell—decreases during differentiation. CytoTRACE outperformed other methods in several test cases and was successfully applied to study cellular hierarchies in healthy and tumor tissue. Science , this issue p. 405 |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| DOI: |
10.1126/science.aax0249 |
| Availability: |
https://doi.org/10.1126/science.aax0249; https://syndication.highwire.org/content/doi/10.1126/science.aax0249; https://www.science.org/doi/pdf/10.1126/science.aax0249 |
| Accession Number: |
edsbas.CE79C535 |
| Database: |
BASE |