| Title: |
A New Derivative of Retro-2 Displays Antiviral Activity against Respiratory Syncytial Virus |
| Authors: |
Le Rouzic, Adrien; Fix, Jenna; Vinck, Robin; Kappler-Gratias, Sandrine; Volmer, Romain; Gallardo, Franck; Eléouët, Jean-François; Keck, Mathilde; Cintrat, Jean-Christophe; Barbier, Julien; Gillet, Daniel; Galloux, Marie |
| Contributors: |
Virologie et Immunologie Moléculaires (VIM (UR 0892)); Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Université Paris-Saclay-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE); Service d'Ingénierie Moléculaire pour la Santé (ex SIMOPRO) (SIMoS); Médicaments et Technologies pour la Santé (MTS); Université Paris-Saclay-Institut des Sciences du Vivant Frédéric JOLIOT (JOLIOT); Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Paris-Saclay-Institut des Sciences du Vivant Frédéric JOLIOT (JOLIOT); Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE); Service de Chimie Bio-Organique et de Marquage (SCBM); NeoVirTech SAS; Interactions hôtes-agents pathogènes Toulouse (IHAP); École nationale vétérinaire de Toulouse (ENVT); Institut National Polytechnique (Toulouse) (Toulouse INP); Communauté d'universités et établissements de Toulouse (Comue de Toulouse)-Communauté d'universités et établissements de Toulouse (Comue de Toulouse)-Institut National Polytechnique (Toulouse) (Toulouse INP); Communauté d'universités et établissements de Toulouse (Comue de Toulouse)-Communauté d'universités et établissements de Toulouse (Comue de Toulouse)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE); This research was funded by the joint ministerial program of R&D against CBRNE risks, CEA, and under Contract ANR-20-CE18-0016 (SMERSEC), ANR-11-IDEX-0003 (PFI n° 20LU1000-131 ReCoVEr and OTP n° 20LU1000_138 PIMVir). A.L.R. was funded by Université Paris-Saclay. The present work has also benefited from Imagerie-Gif core facility supported by l’Agence Nationale de la Recherche (ANR-11-EQPX-0029/Morphoscope, ANR-10-INBS-0004/FranceBioImaging; ANR-11-IDEX-0003/Saclay Plant Sciences).; ANR-11-EQPX-0029,MORPHOSCOPE 2,Imagerie et reconstruction multiéchelles de la morphogenèse. (Plateforme d'innovation technologique et méthodologique pour l'imagerie in vivo et la reconstruction des dynamiques multiéchelles de la morphogenèse)(2011); ANR-10-INBS-0004,France-BioImaging,Développment d'une infrastructure française distribuée coordonnée(2010); ANR-11-IDEX-0003,IPS,Idex Paris-Saclay(2011); ANR-20-CE18-0016,SMERSEC,Structure et fonction de la cible cellulaire du candidat médicament anti-Shiga toxine Retro-2.1(2020) |
| Source: |
ISSN: 1661-6596. |
| Publisher Information: |
CCSD; MDPI |
| Publication Year: |
2024 |
| Collection: |
Université de Versailles Saint-Quentin-en-Yvelines: HAL-UVSQ |
| Subject Terms: |
respiratory syncytial virus; Retro-2.2; fusion protein; retrograde transport; antiviral; [SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie; [SDV.BA.MVSA]Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health; [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology; [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology |
| Description: |
International audience ; Human respiratory syncytial virus (hRSV) is the most common cause of bronchiolitis and pneumonia in newborns, with all children being infected before the age of two. Reinfections are very common throughout life and can cause severe respiratory infections in the elderly and immunocompromised adults. Although vaccines and preventive antibodies have recently been licensed for use in specific subpopulations of patients, there is still no therapeutic treatment commonly available for these infections. Here, we investigated the potential antiviral activity of Retro-2.2, a derivative of the cellular retrograde transport inhibitor Retro-2, against hRSV. We show that Retro-2.2 inhibits hRSV replication in cell culture and impairs the ability of hRSV to form syncytia. Our results suggest that Retro-2.2 treatment affects virus spread by disrupting the trafficking of the viral de novo synthetized F and G glycoproteins to the plasma membrane, leading to a defect in virion morphogenesis. Taken together, our data show that targeting intracellular transport may be an effective strategy against hRSV infection. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| Relation: |
https://doi.org/10.3390/ijms25010415/s1; info:eu-repo/semantics/altIdentifier/pmid/38203585; PUBMED: 38203585; WOS: 001140688200001 |
| DOI: |
10.3390/ijms25010415 |
| Availability: |
https://hal.inrae.fr/hal-04408762; https://hal.inrae.fr/hal-04408762v1/document; https://hal.inrae.fr/hal-04408762v1/file/ijms-25-00415.pdf; https://doi.org/10.3390/ijms25010415 |
| Rights: |
https://creativecommons.org/licenses/by-nc/4.0/ ; info:eu-repo/semantics/OpenAccess |
| Accession Number: |
edsbas.CF0EBB9D |
| Database: |
BASE |