| Title: |
Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition) |
| Authors: |
Cossarizza, Andrea; Chang, Hyun‐Dong; Radbruch, Andreas; Abrignani, Sergio; Addo, Richard; Akdis, Mübeccel; Andrä, Immanuel; Andreata, Francesco; Annunziato, Francesco; Arranz, Eduardo; Bacher, Petra; Bari, Sudipto; Barnaba, Vincenzo; Barros‐Martins, Joana; Baumjohann, Dirk; Beccaria, Cristian G.; Bernardo, David; Boardman, Dominic A.; Borger, Jessica; Böttcher, Chotima; Brockmann, Leonie; Burns, Marie; Busch, Dirk H.; Cameron, Garth; Cammarata, Ilenia; Cassotta, Antonino; Chang, Yinshui; Chirdo, Fernando Gabriel; Christakou, Eleni; Čičin‐Šain, Luka; Cook, Laura; Corbett, Alexandra J.; Cornelis, Rebecca; Cosmi, Lorenzo; Davey, Martin S.; De Biasi, Sara; De Simone, Gabriele; del Zotto, Genny; Delacher, Michael; Di Rosa, Francesca; Di Santo, James; Diefenbach, Andreas; Dong, Jun; Dörner, Thomas; Dress, Regine J.; Dutertre, Charles‐Antoine; Eckle, Sidonia B. G.; Eede, Pascale; Evrard, Maximilien; Falk, Christine S.; Feuerer, Markus; Fillatreau, Simon; Fiz‐Lopez, Aida; Follo, Marie; Foulds, Gemma A.; Fröbel, Julia; Gagliani, Nicola; Galletti, Giovanni; Gangaev, Anastasia; Garbi, Natalio; Garrote, José Antonio; Geginat, Jens; Gherardin, Nicholas A.; Gibellini, Lara; Ginhoux, Florent; Godfrey, Dale I.; Gruarin, Paola; Haftmann, Claudia; Hansmann, Leo; Harpur, Christopher M.; Hayday, Adrian C.; Heine, Guido; Hernández, Daniela Carolina; Herrmann, Martin; Hoelsken, Oliver; Huang, Qing; Huber, Samuel; Huber, Johanna E.; Huehn, Jochen; Hundemer, Michael; Hwang, William Y. K.; Iannacone, Matteo; Ivison, Sabine M.; Jäck, Hans‐Martin; Jani, Peter K.; Keller, Baerbel; Kessler, Nina; Ketelaars, Steven; Knop, Laura; Knopf, Jasmin; Koay, Hui‐Fern; Kobow, Katja; Kriegsmann, Katharina; Kristyanto, H.; Krueger, Andreas; Kuehne, Jenny F.; Kunze‐Schumacher, Heike; Kvistborg, Pia; Kwok, Immanuel; Latorre, Daniela; Lenz, Daniel; Levings, Megan K.; Lino, Andreia C.; Liotta, Francesco; Long, Heather M.; Lugli, Enrico; MacDonald, Katherine N.; Maggi, Laura; Maini, Mala K.; Mair, Florian; Manta, Calin; Manz, Rudolf Armin; Mashreghi, Mir‐Farzin; Mazzoni, Alessio; McCluskey, James; Mei, Henrik E.; Melchers, Fritz; Melzer, Susanne; Mielenz, Dirk; Monin, Leticia; Moretta, Lorenzo; Multhoff, Gabriele; Muñoz, Luis Enrique; Muñoz‐Ruiz, Miguel; Muscate, Franziska; Natalini, Ambra; Neumann, Katrin; Ng, Lai Guan; Niedobitek, Antonia; Niemz, Jana; Almeida, Larissa Nogueira; Notarbartolo, Samuele; Ostendorf, Lennard; Pallett, Laura J.; Patel, Amit A.; Percin, Gulce Itir; Peruzzi, Giovanna; Pinti, Marcello; Pockley, A. Graham; Pracht, Katharina; Prinz, Immo; Pujol‐Autonell, Irma; Pulvirenti, Nadia; Quatrini, Linda; Quinn, Kylie M.; Radbruch, Helena; Rhys, Hefin; Rodrigo, Maria B.; Romagnani, Chiara; Saggau, Carina; Sakaguchi, Shimon; Sallusto, Federica; Sanderink, Lieke; Sandrock, Inga; Schauer, Christine; Scheffold, Alexander; Scherer, Hans U.; Schiemann, Matthias; Schildberg, Frank A.; Schober, Kilian; Schoen, Janina; Schuh, Wolfgang; Schüler, Thomas; Schulz, Axel R.; Schulz, Sebastian; Schulze, Julia; Simonetti, Sonia; Singh, Jeeshan; Sitnik, Katarzyna M.; Stark, Regina; Starossom, Sarah; Stehle, Christina; Szelinski, Franziska; Tan, Leonard; Tarnok, Attila; Tornack, Julia; Tree, Timothy I. M.; van Beek, Jasper J. P.; van de Veen, Willem; van Gisbergen, Klaas; Vasco, Chiara; Verheyden, Nikita A.; von Borstel, Anouk; Ward‐Hartstonge, Kirsten A.; Warnatz, Klaus; Waskow, Claudia; Wiedemann, Annika; Wilharm, Anneke; Wing, James; Wirz, Oliver; Wittner, Jens; Yang, Jennie H. M.; Yang, Juhao |
| Publication Year: |
2022 |
| Collection: |
TU Berlin: Deposit Once |
| Subject Terms: |
610 Medizin und Gesundheit; flow cytometry; cell sorting; immunological studies; immune cell subsets; guidelines |
| Description: |
The third edition of Flow Cytometry Guidelines provides the key aspects to consider when performing flow cytometry experiments and includes comprehensive sections describing phenotypes and functional assays of all major human and murine immune cell subsets. Notably, the Guidelines contain helpful tables highlighting phenotypes and key differences between human and murine cells. Another useful feature of this edition is the flow cytometry analysis of clinical samples with examples of flow cytometry applications in the context of autoimmune diseases, cancers as well as acute and chronic infectious diseases. Furthermore, there are sections detailing tips, tricks and pitfalls to avoid. All sections are written and peer‐reviewed by leading flow cytometry experts and immunologists, making this edition an essential and state‐of‐the‐art handbook for basic and clinical researchers. ; DFG, 389687267, Kompartimentalisierung, Aufrechterhaltung und Reaktivierung humaner Gedächtnis-T-Lymphozyten aus Knochenmark und peripherem Blut ; DFG, 80750187, SFB 841: Leberentzündungen: Infektion, Immunregulation und Konsequenzen ; EC/H2020/800924/EU/International Cancer Research Fellowships - 2/iCARE-2 ; DFG, 252623821, Die Rolle von follikulären T-Helferzellen in T-Helferzell-Differenzierung, Funktion und Plastizität ; DFG, 390873048, EXC 2151: ImmunoSensation2 - the immune sensory system |
| Document Type: |
article in journal/newspaper |
| File Description: |
application/pdf |
| Language: |
English |
| DOI: |
10.14279/depositonce-15411 |
| Availability: |
https://depositonce.tu-berlin.de/handle/11303/16634; https://doi.org/10.14279/depositonce-15411 |
| Rights: |
https://creativecommons.org/licenses/by/4.0/ |
| Accession Number: |
edsbas.CF66DE14 |
| Database: |
BASE |