| Title: |
Fecal gut microbiota and amino acids as noninvasive diagnostic biomarkers of Pediatric inflammatory bowel disease |
| Authors: |
Vermeer,Eva; Jagt,Jasmijn Z.; Lap,Eline M.; Struys, Eduard A.; Budding,Andries E.; Verhoeven-Duif, Nanda M.; Bosma,Marjolein; van Limbergen,Johan E.; Koot,Bart G.P.; de Jonge, Robert; Benninga,Marc A.; Acharjee,Animesh; de Boer,Nanne K.H.; de Meij,Tim G.J.; Genetica; UMC Utrecht Holding; Cancer; Child Health |
| Publication Year: |
2025 |
| Subject Terms: |
amino acids; gut microbiota; Paediatric inflammatory bowel disease; Microbiology; Microbiology (medical); Gastroenterology; Infectious Diseases |
| Description: |
Background and Aims: Fecal calprotectin (FCP) has limited specificity as diagnostic biomarker of pediatric inflammatory bowel disease (IBD), leading to unnecessary invasive endoscopies. This study aimed to develop and validate a fecal microbiota and amino acid (AA)-based diagnostic model. Methods: Fecal samples from a discovery cohort (de novo IBD and healthy controls [HC]) were used to develop the diagnostic model. This model was applied in a validation cohort (de novo IBD and controls with gastrointestinal symptoms [CGI]). Microbiota and AAs were analyzed using interspace profiling and liquid chromatography-mass spectrometry techniques, respectively. Machine learning techniques were used to build the diagnostic model. Results: In the discovery cohort (58 IBD, 59 hC), two microbial species (Escherichia coli and Alistipes finegoldii) and four AAs (leucine, ornithine, taurine, and alpha-aminoadipic acid [AAD]) combined allowed for discrimination between both subgroups (AUC 0.94, 95% CI [0.89, 0.98]). In the validation cohort (43 IBD, 38 CGI), this panel of six markers could differentiate patients with IBD from CGI with an AUC of 0.84, 95% CI [0.67, 0.95]). Leucine showed the best diagnostic performance (AUC 0.89, 95% CI [0.81, 0.95]). Conclusions: Leucine might serve as adjuvant noninvasive biomarker in the diagnostic work-up of pediatric IBD. Future research should investigate whether the combination of leucine with FCP could improve specificity and may help tailor the course of diagnostics. |
| Document Type: |
article in journal/newspaper |
| File Description: |
application/pdf |
| Language: |
English |
| ISSN: |
1949-0976 |
| Relation: |
https://dspace.library.uu.nl/handle/1874/466668 |
| Availability: |
https://dspace.library.uu.nl/handle/1874/466668 |
| Rights: |
info:eu-repo/semantics/OpenAccess |
| Accession Number: |
edsbas.CF6E9518 |
| Database: |
BASE |