| Title: |
Sex-specific autophagy modulation in osteoblastic lineage: a critical function to counteract bone loss in female. |
| Authors: |
Camuzard, Olivier; Santucci-Darmanin, Sabine; Breuil, Véronique; Cros, Chantal; Gritsaenko, Tatiana; Pagnotta, Sophie; Cailleteau, Laurence; Battaglia, Séverine; Panaïa-Ferrari, Patricia; Heymann, Dominique; Carle, Georges, F; Pierrefite-Carle, Valérie |
| Contributors: |
Transporteurs en Imagerie et Radiothérapie en Oncologie - Mécanismes biologiques des Altérations du Tissu Osseux (TIRO-MATOs); Service Hospitalier Frédéric Joliot (SHFJ); Université Paris-Saclay-Institut des Sciences du Vivant Frédéric JOLIOT (JOLIOT); Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Institut des Sciences du Vivant Frédéric JOLIOT (JOLIOT); Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Nice Sophia Antipolis (1965 - 2019) (UNS)-Université Côte d'Azur (UniCA); Service de Chirurgie Réparatrice et de la Main CHU de Nice; Centre Hospitalier Universitaire de Nice (CHU Nice); Service de rhumatologie; Centre Hospitalier Universitaire de Nice (CHU Nice)-Hôpital l'Archet; Centre Commun de Microscopie Appliquée (CCMA); Université Côte d'Azur (UniCA); Institut de Recherche sur le Cancer et le Vieillissement (IRCAN); Université Nice Sophia Antipolis (1965 - 2019) (UNS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Côte d'Azur (UniCA); Physiopathologie des Adaptations Nutritionnelles (PhAN); Institut National de la Recherche Agronomique (INRA)-Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE); Université de Nantes (UN)-Université de Nantes (UN); Laboratoire de Biochimie CHU Nice; Department of Oncology and Metabolism Sheffield, UK (European Associated Laboratory “Sarcoma Research Unit”); University of Sheffield Sheffield; This work was supported by the Commissariat à l’Energie Atomique et aux énergies alternatives (CEA) and the Société Française de Rhumatologie (SFR). |
| Source: |
ISSN: 1949-2553 ; Oncotarget ; https://inserm.hal.science/inserm-01701035 ; Oncotarget, 2016, 7 (41), pp.66416-66428. ⟨10.18632/oncotarget.12013⟩. |
| Publisher Information: |
CCSD; Impact journals |
| Publication Year: |
2016 |
| Collection: |
Institut National de la Recherche Agronomique: ProdINRA |
| Subject Terms: |
aging; Gerotarget; osteoporosis; autophagy; osteoblast; osteocyte; [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology; [SDV.MHEP.RSOA]Life Sciences [q-bio]/Human health and pathology/Rhumatology and musculoskeletal system |
| Description: |
International audience ; Age-related bone loss is associated with an increased oxidative stress which is worsened by estrogen fall during menauposis. This observation has drawn attention to autophagy, a major cellular catabolic process, able to alleviate oxidative stress in osteoblasts (OB) and osteocytes (OST), two key bone cell types. Moreover, an autophagy decline can be associated with aging, suggesting that an age-related autophagy deficiency in OB and/or OST could contribute to skeletal aging and osteoporosis onset. In the present work, autophagy activity was analyzed in OST and OB in male and female mice according to their age and hormonal status. In OST, autophagy decreases with aging in both sexes. In OB, although a 95% decrease in autophagy is observed in OB derived from old females, this activity remains unchanged in males. In addition, while ovariectomy has no effect on OB autophagy levels, orchidectomy appears to stimulate this process. An inverse correlation between autophagy and the oxidative stress level was observed in OB derived from males or females. Finally, using OB-specific autophagy-deficient mice, we showed that autophagy deficiency aggravates the bone loss associated with aging and estrogen deprivation. Taken together, our data indicate that autophagic modulation in bone cells differs according to sex and cell type. The lowering of autophagy in female OB, which is associated with an increased oxidative stress, could play a role in osteoporosis pathophysiology and suggests that autophagy could be a new therapeutic target for osteoporosis in women. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| Relation: |
info:eu-repo/semantics/altIdentifier/pmid/27634908; PUBMED: 27634908; PUBMEDCENTRAL: PMC5341810 |
| DOI: |
10.18632/oncotarget.12013 |
| Availability: |
https://inserm.hal.science/inserm-01701035; https://inserm.hal.science/inserm-01701035v1/document; https://inserm.hal.science/inserm-01701035v1/file/camuzard%20o%20et%20al_sex%20specific%20autophagy_oncotarget.pdf; https://doi.org/10.18632/oncotarget.12013 |
| Rights: |
https://about.hal.science/hal-authorisation-v1/ ; info:eu-repo/semantics/OpenAccess |
| Accession Number: |
edsbas.D01E9EB9 |
| Database: |
BASE |