Guidelines for the use and interpretation of assays for monitoring autophagy in higher eukaryotes
| Title: | Guidelines for the use and interpretation of assays for monitoring autophagy in higher eukaryotes |
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| Authors: | KLIONSKY DJ; ABELIOVICH H; AGOSTINIS P; AGRAWAL DK; ALIEV G; ASKEW DS; BABA M; BAEHRECKE EH; BAHR BA; BALLABIO A; BAMBER BA; BASSHAM DC; BERGAMINI E; BI X; BIARD PIECHACZYK M; BLUM JS; BREDESEN DE; BRODSKY JL; BRUMELL JH; BRUNK UT; BURSCH W; CAMOUGRAND N; CEBOLLERO E; CECCONI F; CHEN Y; CHIN LS; CHOI A; CHU CT; CHUNG J; CLARKE PG; CLARK RS; CLARKE SG; CLAVÉ C; CLEVELAND JL; CODOGNO P; COLOMBO MI; COTO MONTES A; CREGG JM; CUERVO AM; DEBNATH J; DEMARCHI F; DENNIS PB; DENNIS PA; DERETIC V; DEVENISH RJ; DI SANO F; DICE JF; DIFIGLIA M; DINESH KUMAR S; DISTELHORST CW; DJAVAHERI MERGNY M; DORSEY FC; DRÖGE W; DRON M; DUNN WA JR; DUSZENKO M; EISSA NT; ELAZAR Z; ESCLATINE A; ESKELINEN EL; FÉSÜS L; FINLEY KD; FUENTES JM; FUEYO J; FUJISAKI K; GALLIOT B; GAO FB; GEWIRTZ DA; GIBSON SB; GOHLA A; GOLDBERG AL; GONZALEZ R; GONZÁLEZ ESTÉVEZ C; GORSKI S; GOTTLIEB RA; HÄUSSINGER D; HE YW; HEIDENREICH K; HILL JA; HØYER HANSEN M; HU X; HUANG WP; IWASAKI A; JÄÄTTELÄ M; JACKSON WT; JIANG X; JIN S; JOHANSEN T; JUNG JU; KADOWAKI M; KANG C; KELEKAR A; KESSEL DH; KIEL JA; KIM HP; KIMCHI A; KINSELLA TJ; KISELYOV K; KITAMOTO K; KNECHT E; KOMATSU M; KOMINAMI E; KONDO S; KOVÁCS AL; KROEMER G; KUAN CY; KUMAR R; KUNDU M; LANDRY J; LAPORTE M; LE W; LEI HY; LENARDO MJ; LEVINE B; LIEBERMAN A; LIM KL; LIN FC; LIOU W; LIU LF; LOPEZ BERESTEIN G; LÓPEZ OTÍN C; LU B; MACLEOD KF; MALORNI W; MARTINET W; MATSUOKA K; MAUTNER J; MEIJER AJ; MELÉNDEZ A; MICHELS P; MISTIAEN WP; MIZUSHIMA N; MOGRABI B; MONASTYRSKA I; MOORE MN; MOREIRA PI; MORIYASU Y; MOTYL T; MÜNZ C; MURPHY LO; NAQVI NI; NEUFELD TP; NISHINO I; NIXON RA; NODA T; NÜRNBERG B; OGAWA M; OLEINICK NL; OLSEN LJ; OZPOLAT B; PAGLIN S; PALMER GE; PAPASSIDERI I; PARKES M; PERLMUTTER DH; PERRY G; PIACENTINI M; PINKAS KRAMARSKI R; PRESCOTT M; PROIKAS CEZANNE T; RABEN N; RAMI A; REGGIORI F; ROHRER B; RUBINSZTEIN DC; RYAN KM; SADOSHIMA J; SAKAGAMI H; SAKAI Y; SASAKAWA C; SASS M; SCHNEIDER C; SEGLEN PO; SELEVERSTOV O; SETTLEMAN J; SHACKA JJ; SHAPIRO IM; SIBIRNY A; SILVA ZACARIN EC; SIMON HU; SIMONE C; SIMONSEN A; SMITH MA; SPANEL BOROWSKI K; SRINIVAS V; STEEVES M; STENMARK H; STROMHAUG PE; SUBAUSTE CS; SUGIMOTO S; SULZER D; SUZUKI T; SWANSON MS; TABAS I; TAKESHITA F; TALBOT NJ; TALLÓCZY Z; TANAKA K; TANIDA I; TAYLOR GS; TAYLOR JP; TERMAN A; TETTAMANTI G; THOMPSON CB; THUMM M; TOLKOVSKY AM; TOOZE SA; TRUANT R; TUMANOVSKA LV; UCHIYAMA Y; UENO T; UZCÁTEGUI NL; VAN DER KLEI I; VAQUERO EC; VELLAI T; VOGEL MW; WANG HG; WEBSTER P; WILEY JW; XI Z; XIAO G; YAHALOM J; YANG JM; YAP G; YIN XM; YOSHIMORI T; YU L; YUE Z; YUZAKI M; ZABIRNYK O; ZHENG X; ZHU X; DETER RL; MIOTTO, GIOVANNI; SANDRI, MARCO |
| Contributors: | Klionsky, Dj; Abeliovich, H; Agostinis, P; Agrawal, Dk; Aliev, G; Askew, D; Baba, M; Baehrecke, Eh; Bahr, Ba; Ballabio, A; Bamber, Ba; Bassham, Dc; Bergamini, E; Bi, X; BIARD PIECHACZYK, M; Blum, J; Bredesen, De; Brodsky, Jl; Brumell, Jh; Brunk, Ut; Bursch, W; Camougrand, N; Cebollero, E; Cecconi, F; Chen, Y; Chin, L; Choi, A; Chu, Ct; Chung, J; Clarke, Pg; Clark, R; Clarke, Sg; Clavé, C; Cleveland, Jl; Codogno, P; Colombo, Mi; COTO MONTES, A; Cregg, Jm; Cuervo, Am; Debnath, J; Demarchi, F; Dennis, Pb; Dennis, Pa; Deretic, V; Devenish, Rj; DI SANO, F; Dice, Jf; Difiglia, M; DINESH KUMAR, S; Distelhorst, Cw; DJAVAHERI MERGNY, M; Dorsey, Fc; Dröge, W; Dron, M; DUNN WA, Jr; Duszenko, M; Eissa, Nt; Elazar, Z; Esclatine, A; Eskelinen, El; Fésüs, L; Finley, Kd; Fuentes, Jm; Fueyo, J; Fujisaki, K; Galliot, B; Gao, Fb; Gewirtz, Da; Gibson, Sb; Gohla, A; Goldberg, Al; Gonzalez, R; GONZÁLEZ ESTÉVEZ, C; Gorski, S; Gottlieb, Ra; Häussinger, D; He, Yw; Heidenreich, K; Hill, Ja; HØYER HANSEN, M; Hu, X; Huang, Wp; Iwasaki, A; Jäättelä, M; Jackson, Wt; Jiang, X; Jin, S; Johansen, T; Jung, Ju; Kadowaki, M; Kang, C; Kelekar, A; Kessel, Dh; Kiel, Ja; Kim, Hp; Kimchi, A; Kinsella, Tj; Kiselyov, K; Kitamoto, K; Knecht, E |
| Publication Year: | 2008 |
| Collection: | Padua Research Archive (IRIS - Università degli Studi di Padova) |
| Subject Terms: | autolysosome; autophagosome; phagophore; autophagy |
| Description: | Research in autophagy continues to accelerate,1 and as a result many new scientists are entering the field. Accordingly, it is important to establish a standard set of criteria for monitoring macroautophagy in different organisms. Recent reviews have described the range of assays that have been used for this purpose.(2,3) There are many useful and convenient methods that can be used to monitor macroautophagy in yeast, but relatively few in other model systems, and there is much confusion regarding acceptable methods to measure macroautophagy in higher eukaryotes. A key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers of autophagosomes versus those that measure flux through the autophagy pathway; thus, a block in macroautophagy that results in autophagosome accumulation needs to be differentiated from fully functional autophagy that includes delivery to, and degradation within, lysosomes (in most higher eukaryotes) or the vacuole (in plants and fungi). Here, we present a set of guidelines for the selection and interpretation of the methods that can be used by investigators who are attempting to examine macroautophagy and related processes, as well as by reviewers who need to provide realistic and reasonable critiques of papers that investigate these processes. This set of guidelines is not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to verify an autophagic response. |
| Document Type: | article in journal/newspaper |
| File Description: | STAMPA |
| Language: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/18188003; info:eu-repo/semantics/altIdentifier/wos/WOS:000253008800003; volume:4; firstpage:151; lastpage:175; numberofpages:25; journal:AUTOPHAGY; https://hdl.handle.net/11577/2440714 |
| Availability: | https://hdl.handle.net/11577/2440714 |
| Rights: | info:eu-repo/semantics/openAccess ; license:Accesso gratuito |
| Accession Number: | edsbas.D04CB500 |
| Database: | BASE |