| Title: |
Dysfunctional Adipocytes in Cardiovascular Biology |
| Authors: |
Giannetta, E; Fiore, D; Gianfrilli, D; Galea, N; Di Dato, C; Pofi, R; Pozza, C; Sbardella, E; Carbone, I; Naro, F; Lenzi, A; Venneri, M; Isidori, AM; Zaborska, KE; Edwards, G; Austin, C; Wareing, M; Massaro, M; Scoditti, E; Pellegrino, M; Carluccio, MA; Calabriso, N; Wabitsch, M; Storelli, C; Wright, M; De Caterina, R |
| Publisher Information: |
Oxford University Press |
| Publication Year: |
2016 |
| Collection: |
HighWire Press (Stanford University) |
| Subject Terms: |
Saturday 9 July 2016 |
| Description: |
Background: Visceral adipose plays a significant role in cardiovascular risk. PDE5 inhibitors (PDE5i) can improve cardiac function and insulin sensitivity in type 2 diabetic patients (T2DM). Purpose: To investigate whether PDE5i affect visceral adipose tissue (VAT), specifically epicardial fat (EAT), and what the mechanism involved using microarray-based profiling of pharmacologically modulated miRNAs. Methods: a randomized, double-blid, placebo-controlled study was designed in T2DM. 59 diabetic patients were randomized to receive 100 mg/d Sildenafil or Placebo for 12 weeks. Fat biopses were performed in a subgroup of patients. In a parallel animal study, db/db mice were randomized to 12.week Sildenafil or vehicle and VAT was collected. Main outcomes and measures were anthropometric and metabolic parameters, EAT quantification through cardiac magnetic resonance imaging (CMR), array of circulating 2005 miRNAs, qPCR and flow cytometry of VAT. Results: Compared to Placebo, Sildenafil reduced waist circumference (p=0.024) an EAT by CMR (p =0.045). Microarray analysis identified some miRNAs differentially regulate by Sildenafil, among which a downregulation of miR-22-3p, confirmed by real-time qPCR (p |
| Document Type: |
text |
| File Description: |
text/html |
| Language: |
English |
| Relation: |
http://cardiovascres.oxfordjournals.org/cgi/content/short/111/suppl_1/S45; http://dx.doi.org/10.1093/cvr/cvw126 |
| DOI: |
10.1093/cvr/cvw126 |
| Availability: |
http://cardiovascres.oxfordjournals.org/cgi/content/short/111/suppl_1/S45; https://doi.org/10.1093/cvr/cvw126 |
| Rights: |
Copyright (C) 2016, European Society of Cardiology |
| Accession Number: |
edsbas.D1255A7 |
| Database: |
BASE |