| Title: |
Loss of Function in Dopamine Receptor‐3 (D3R) Attenuates Left Ventricular Cardiac Fibroblast Migration and Proliferation In Vitro |
| Authors: |
Kisling, Andrew; Zakari, Madaniah O; Alsahly, Musaad B; Melit‐Thomas, Deepthy C; Clemens, Stefan; Lust, Robert M; Katwa, Laxmansa C |
| Source: |
The FASEB Journal ; volume 33, issue S1 ; ISSN 0892-6638 1530-6860 |
| Publisher Information: |
Wiley |
| Publication Year: |
2019 |
| Collection: |
Wiley Online Library (Open Access Articles via Crossref) |
| Description: |
Dopamine is a neurotransmitter heavily involved in neural pathways regulating the reward response, body movement, mood, and cognitive function. As such, much of the research on dopamine and its five receptor subtypes has been conducted to observe the in vivo function of each receptor subtype in neurological disorders. However, recent studies by colleagues from our institution have suggested that D3Rs may play a role in cardiac‐related aging, as 2‐month old D3 receptor knock‐out (D3KO) mice show age‐related changes in cardiac function similar to 2‐year old wild‐type (WT) mice. Thus, it would be interesting to understand the role of D3R expression in LV cardiac fibroblast and its function. We isolated, cultured and characterized cardiac fibroblasts from the left ventricles (LV) of WT (23‐weeks old) and D3KO (5 and 31‐week‐old) mice. Immunohistochemistry was performed to determine the expression of D3R in LV cardiac fibroblasts, and cell culture experiments were performed to examine possible changes in WT and D3KO cell migration and proliferation. Proliferation was measured via total cell count over time points of 6, 12, 24, and 36 hours, while migration in response to scratch wound was examined as percent change in area at 3, 6, 12, 24 hours, and every 12 hours thereafter up to 86 hours. Staining with antibodies for D3R successfully showed expression of this receptor in WT fibroblasts, while cell culture experiments showed reduced proliferation and migration of D3KO fibroblasts compared to WT. Migration of D3KO fibroblasts from 5‐week old mice were significantly different (*p |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| DOI: |
10.1096/fasebj.2019.33.1_supplement.706.2 |
| Availability: |
https://doi.org/10.1096/fasebj.2019.33.1_supplement.706.2 |
| Rights: |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
| Accession Number: |
edsbas.D14F666C |
| Database: |
BASE |