| Title: |
AuNPs amplification in surface plasmon resonance imaging for the detection of micro-RNAs in the context of organ donation |
| Authors: |
Beltrami, Coline; Moreau, Julien; Convert, L.; Bryche, Jean-François; Charette, Paul; Canva, Michael |
| Contributors: |
Institut Interdisciplinaire d'Innovation Technologique Sherbrooke (3IT); Université de Sherbrooke = University of Sherbrooke Sherbrooke (UdeS); Laboratoire Nanotechnologies et Nanosystèmes Sherbrooke (LN2); Université de Sherbrooke = University of Sherbrooke Sherbrooke (UdeS)-École Centrale de Lyon (ECL); Université de Lyon-Université de Lyon-École Supérieure de Chimie Physique Électronique de Lyon (CPE)-Institut National des Sciences Appliquées de Lyon (INSA Lyon); Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA); Laboratoire Charles Fabry / Biophotonique; Laboratoire Charles Fabry (LCF); Institut d'Optique Graduate School (IOGS)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Institut d'Optique Graduate School (IOGS)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS) |
| Source: |
Plasmonics in Biology and Medicine XXI, SPIE Photonics West, proceedings SPIE ; https://hal.science/hal-04654878 ; Plasmonics in Biology and Medicine XXI, SPIE Photonics West, proceedings SPIE, Jan 2024, San Francisco CA, United States. pp.PC128600C, ⟨10.1117/12.2692873⟩ |
| Publisher Information: |
CCSD |
| Publication Year: |
2024 |
| Collection: |
Université de Lyon: HAL |
| Subject Terms: |
[SPI]Engineering Sciences [physics] |
| Subject Geographic: |
San Francisco CA; United States |
| Description: |
International audience ; We present a Surface Plasmon Resonance Imaging (SPRI) biochip system to quantitatively detect micro-RNAs involved in the cytokine storm during an inflammatory response. The thiol composition of the self-assembled monolayer on the biochip gold surface was tuned to maximize the capture of RNAs at low concentrations. To further amplify this signal, we have developed a sandwich-like assay using oligonucleotides functionalized gold nanoparticles (AuNPs), synthesized at ambient temperature and optimized to have a high solubility in saline solutions. Sub-picomolar detection limit of those small RNAs was achieved with all these combined improvements. |
| Document Type: |
conference object |
| Language: |
English |
| DOI: |
10.1117/12.2692873 |
| Availability: |
https://hal.science/hal-04654878; https://doi.org/10.1117/12.2692873 |
| Accession Number: |
edsbas.D1AAD75E |
| Database: |
BASE |