| Title: |
Evaluating the Genomic Diversity of Rice (Oryza sativa L.): SNP-typing in 11 Early-Backcross Introgression Breeding Populations |
| Authors: |
Jauhar Ali; Muhammad U. Aslam; Rida Tariq; Varunseelan Murugaiyan; Patrick Schnable; Corinne M. Nazarea; Jose Hernandez; Muhammad Arif; Jianlong Xu; Zhikang Li |
| Contributors: |
Labroo, Marlee; Ali, Jauhar |
| Publisher Information: |
Harvard Dataverse |
| Publication Year: |
2018 |
| Collection: |
Harvard Dataverse Network |
| Subject Terms: |
Agricultural Sciences; single nucleotide polymorphism; genotyping by sequencing; Oryza sativa; introgression; SNP-typing; tunable Genotyping-by-Sequencing (tGBS®); Genotyping-by-Sequencing; non-synonymous SNP; marker-assisted breeding (selection) |
| Description: |
This study demonstrates GBS based SNP-typing in 11 early-backcross introgression populations of rice (at BC1F5), comprising a set of 564 diverse introgression lines and 12 parents. Sequencing using 10 Ion Proton runs generated a total of ~943.4 million raw reads, out of which ~881.6 million reads remained after trimming for low-quality bases. After alignment, 794,297 polymorphic SNPs were identified, and filtering resulted in LMD50 SNPs (low missing data, with each SNP, genotyped in at least 50% of the samples) for each sub-population. Every data point was supported by actual sequencing data without any imputation, eliminating imputation-induced errors in SNP-calling. Genotyping substantiated the impacts of novel breeding strategy revealing: (a) the donor introgression pattern in ILs were characteristic with variable introgression frequency in different genomic regions, attributed mainly to stringent selection under abiotic stress (b) considerably lower heterozygosity was observed in the ILs. Functional annotation revealed 426 non-synonymous deleterious SNPs present in 102 loci with a range of 1 to 4 SNPs per locus and 120 novel SNPs. SNP-typing this diversity panel will further assist in the development of markers supporting genomic applications in molecular breeding programs. Raw sequencing data is available at the NCBI SRA PRJNA479931. |
| Document Type: |
dataset |
| Language: |
English |
| Relation: |
https://doi.org/10.7910/DVN/RRXCR3 |
| DOI: |
10.7910/DVN/RRXCR3 |
| Availability: |
https://doi.org/10.7910/DVN/RRXCR3 |
| Accession Number: |
edsbas.D2A5EA7F |
| Database: |
BASE |