| Publisher Information: |
Uppsala universitet, Institutionen för immunologi, genetik och patologi; Japan Med Device Startup Incubat Program, R&D Dept, 3-7-2 Nihonbashihon Cho,Chuo Ku, Tokyo 1030023, Japan.;NB Med Inc, R&D Dept, 3-7-2 Nihonbashihon Cho,Chuo Ku, Tokyo 1030023, Japan.; NB Med Inc, R&D Dept, 3-7-2 Nihonbashihon Cho,Chuo Ku, Tokyo 1030023, Japan.; Univ Calif Los Angeles, Ronald Reagan UCLA Med Ctr, David Geffen Sch Med, Div Intervent Neuroradiol,Dept Radiol Sci, 757 Westwood Plaza,Suite 2129, Los Angeles, CA 90095 USA.; Univ Tokyo, Grad Sch Engn, Dept Bioengn, 7-3-1 Hongo,Bunkyo Ku, Tokyo 1138656, Japan.; Natl Inst Adv Ind Sci & Technol, Cellular & Mol Biotechnol Res Inst CMB, AIST Tsukuba Cent 5,1-1-1 Higashi, Tsukuba, Ibaraki 3058565, Japan.; Osaka Univ, Grad Sch Engn, Div Mat & Mfg Sci, 2-1 Yamadaoka, Suita, Osaka 5650871, Japan.; Natl Inst Adv Ind Sci & Technol, Cellular & Mol Biotechnol Res Inst CMB, AIST Tsukuba Cent 5,1-1-1 Higashi, Tsukuba, Ibaraki 3058565, Japan.;Univ Tsukuba, Masters Doctoral Program Life Sci Innovat T LSI, 1-1-1 Tennodai, Tsukuba, Ibaraki 3058577, Japan. |
| Description: |
The advent of intracranial stents has revolutionized the endovascular treatment of cerebral aneurysms. The utilization of stents has rendered numerous cerebral aneurysm amenable to endovascular treatment, thereby obviating the need for otherwise invasive open surgical options. Stent placement has become a mainstream approach because of its safety and efficacy. However, further improvements are required for clinically approved devices to avoid the frequent occurrence of thrombotic complications. Therefore, controlling the thrombotic complications associated with the use of devices is of significant importance. Our group has developed a unique stent coated with a 2-methacryloyloxyethyl phosphorylcholine (MPC)-based polymer. In this study, the surface characteristics of the polymer coating were verified using X-ray photoelectron spectroscopy and atomic force microscopy. Subsequently, the antithrombotic properties of the coating were evaluated by measuring platelet count and thrombin-antithrombin complex levels of whole human blood after 3 h of incubation in a Chandler loop model. Scanning electron microscopy was utilized to examine thrombus formation on the stent surface. We observed that MPC polymer-coated stents significantly reduced thrombus formation as compared to bare stents and several clinically approved devices. Finally, the coated stents were further analyzed by implanting them in the internal thoracic arteries of pigs. Angiographic imaging and histopathological examinations that were performed one week after implantation revealed that the vascular lumen was well maintained and coated stents were integrated within the vascular endothelium without inducing adverse effects. Thus, we demonstrated the efficacy of MPC polymer coating as a viable strategy for avoiding the thrombotic risks associated with neurovascular stents. |