| Title: |
In vivo imaging of retrovirus infection reveals a role for Siglec-1/CD169 in multiple routes of transmission |
| Authors: |
Uchil, Pradeep; Haugh, Kelsey; Ladinsky, Mark; Ullah, Irfan; Stone, Helen; Pi, Ruoxi; Gilardet, Alexandre; Grunst, Michael; Kumar, Priti; Bjorkman, Pamela; Mothes, Walther |
| Publisher Information: |
Zenodo |
| Publication Year: |
2021 |
| Collection: |
Zenodo |
| Subject Terms: |
confocal imaging; CD169; Macrophages; Murine Leukemia Virus; Oral route; subcutaneous infection; Peyer's patches; Gag-GFP |
| Description: |
Early events in retrovirus transmission are determined by interactions between incoming viruses and frontline cells near entry sites. Despite their importance for retroviral pathogenesis, very little is known about these events. We developed a bioluminescence imaging (BLI)-guided multiscale imaging approach to study these events in vivo. Engineered murine leukemia reporter viruses allowed us to monitor individual stages of retrovirus life cycle including virus particle flow, virus entry into cells, infection and spread for retroorbital, subcutaneous and oral routes. BLI permitted temporal tracking of orally administered retroviruses along the gastrointestinal tract as they traversed the lumen through Peyer's Patches to reach the draining mesenteric sac. Importantly, capture and acquisition of lymph-, blood- and milk-borne retroviruses spanning three routes, was promoted by a common host factor, the I-type lectin CD169, expressed on sentinel macrophages. These results highlight how retroviruses co-opt the immune surveillance function of tissue resident sentinel macrophages for establishing infection. ; Related to Figure 4 in the manuscript: Sentinel macrophages mediate capture of incoming viruses at the injection site during subcutaneous challenge. Related to Figure 5 in the manucript: Visualization of MLV transmission from mother to offspring. Related to Figure 8 in the manucript: Gut-infiltrating MLV is captured by Peyer's Patch-resident CD169+ macrophages Related to Figure 9 in the manucript: CD169 contributes to retrovirus particle acquisition and establishment of infection during oral challenge Funding provided by: National Institute of Allergy and Infectious DiseasesCrossref Funder Registry ID: http://dx.doi.org/10.13039/100000060Award Number: R01 CA098727 Funding provided by: National Institute of Allergy and Infectious DiseasesCrossref Funder Registry ID: http://dx.doi.org/10.13039/100000060Award Number: P50AI150464 Funding provided by: National Institute of Allergy and Infectious DiseasesCrossref Funder ... |
| Document Type: |
dataset |
| Language: |
unknown |
| Relation: |
https://zenodo.org/communities/dryad/; https://zenodo.org/records/5790253; oai:zenodo.org:5790253; https://doi.org/10.5061/dryad.hhmgqnkgw |
| DOI: |
10.5061/dryad.hhmgqnkgw |
| Availability: |
https://doi.org/10.5061/dryad.hhmgqnkgw; https://zenodo.org/records/5790253 |
| Rights: |
Creative Commons Zero v1.0 Universal ; cc0-1.0 ; https://creativecommons.org/publicdomain/zero/1.0/legalcode |
| Accession Number: |
edsbas.D5630CEA |
| Database: |
BASE |