| Title: |
β-glucan imprinting remodels macrophage function in response to environmental cues |
| Authors: |
Piffer, Alícia, C; Camilli, Giorgio; Bohm, Mathieu; Lavenir, Rachel; Quintin, Jessica |
| Contributors: |
Immunologie des Infections fongiques - Immunology of fungal infections; Institut Pasteur Paris (IP)-Université Paris Cité (UPCité); Instituto de Microbiologia Paulo de Góes Rio de Janeiro (IMPG); Universidade Federal do Rio de Janeiro Brasil = Federal University of Rio de Janeiro Brazil = Université fédérale de Rio de Janeiro Brésil (UFRJ); A.C.P, G.C., M.B., R.L., and J.Q. were supported by the ANR JCJC grant (ANR-16-CE15-0014-01), and by Institut Carnot–Microbes et Santé grant (n°11 CARN-017-01) (to J.Q.). The authors also acknowledge funding from CAPES finance code 001. A.C.P. was supported by the CAPES-COFECUB Franco-Brazilian Research Exchange Program (88887.281641/2018-00).For the purpose of open access, the author has applied a CC BY public copyright license to any author accepted manuscript version arising from this submission; ANR-16-CE15-0014,IIMory,Mémoire immunologique innée des défenses de l'hôte(2016) |
| Source: |
https://pasteur.hal.science/pasteur-03712988 ; 2022. |
| Publisher Information: |
CCSD |
| Publication Year: |
2022 |
| Subject Terms: |
[SDV]Life Sciences [q-bio] |
| Description: |
Posté sur BioRxiv le 6 déc 2021 ; Abstract In vitro , exposure of human primary monocytes to the fungal β-glucan enhances their pro-inflammatory responsiveness towards several pathogens. Yet, the role of environmental condition of this process remains unclear. Here we found that β-glucan-induced innate immune memory counteract the anti-inflammatory status of the macrophages. In response to β-glucan imprinting, M-CSF-(M2-like-) macrophages increase their pro-inflammatory responsiveness to secondary stimuli associated with decrease of the M-CSF differentiation hallmarks. In contrast, in GM-CSF-(M1-like-) environment, β-glucan imprinting reduced the pro-inflammatory canonical feature of the macrophages, together with their terminal differentiation marks. Comparing M-CSF and GM-CSF environment, we observed that β-glucan-imprinted macrophages present comparable functions in terms of cytokine responses, phagocytosis, oxidative burst, and angiogenesis. This effect is mediated through Dectin-1 and associated with altered expression of the master regulators of macrophage terminal differentiation, IRF5 and IRF3. β-glucan-induced innate immune memory skews the commitment of the macrophages in complex environment towards similar and less terminally differentiated cells. Together, these observations suggest a potential therapeutic role for β-glucan-induced modulation of innate memory in different pathological contexts. |
| Document Type: |
report |
| Language: |
English |
| Relation: |
BIORXIV: 2021.08.30.458241 |
| DOI: |
10.1101/2021.08.30.458241 |
| Availability: |
https://pasteur.hal.science/pasteur-03712988; https://pasteur.hal.science/pasteur-03712988v1/document; https://pasteur.hal.science/pasteur-03712988v1/file/BIORXIV-2021-458241v2-Quintin.pdf; https://doi.org/10.1101/2021.08.30.458241 |
| Rights: |
http://creativecommons.org/licenses/by-nc-nd/ ; info:eu-repo/semantics/OpenAccess |
| Accession Number: |
edsbas.D5CD52FE |
| Database: |
BASE |