| Title: |
Developmental Basis of Cardiac Inherited Diseases |
| Authors: |
Perez-Pomares, JM; Cano, E; Carmona, R; Ruiz-Villalba, A; Rojas, A; Chau, Y-Y; Wagner, KD; Wagner, N; Hastie, ND; Munoz-Chapuli, R; N/A; Gaertner-Rommel, A; Klauke, B; Linke, WA; Schulz, U; Laser, KT; Gummert, J; Milting, H; Lazzarini, E; Bauce, B; Cason, M; Celeghin, R; Rigato, I; Carturan, E; Rizzo, S; Thiene, G; Basso, C; Pilichou, K |
| Publisher Information: |
Oxford University Press |
| Publication Year: |
2016 |
| Collection: |
HighWire Press (Stanford University) |
| Subject Terms: |
Sunday 10 July 2016 |
| Description: |
Recent reports suggest that mammalian embryonic coronary endothelium (CoE) develops from the sinus venosus and ventricular endocardium. Although several studies in non-mammalian vertebrates have proven that extracardiac endothelial cells also participate in CoE development, the contribution of such cells to mammalian CoE is regarded to be minor and non-significant. Using classic (Wt1Cre) and novel (G2-Gata4Cre) mouse transgenic models for the study of coronary vascular development, we show that extracardiac septum transversum/proepicardiun (ST/PE)-derived endothelial cells are required for proper coronary development. Our results indicate that, at least, 20% of embryonic coronary arterial and capillary endothelial cells derive from the ST/PE. Conditional deletion of the epicardial lineage gene Wt1 in the ST/PE (G2-Gata4Cre) or the endothelium (Tie2Cre) reveals a critical role for these ST/PE-derived endothelial cells in the establishment of transmural ventricular coronary arterio-venous connections. Taken together, our results confirm that embryonic CoE is a developmental mosaic forming from different endothelial sources, and that extracardiac cells are necessary for the completion of coronary morphogenesis. These data contribute to our understanding of some coronary congenital anomalies and suggest embryonic CoE heterogeneity as an ontogenetic factor for adult coronary disease. |
| Document Type: |
text |
| File Description: |
text/html |
| Language: |
English |
| Relation: |
http://cardiovascres.oxfordjournals.org/cgi/content/short/111/suppl_1/S84; http://dx.doi.org/10.1093/cvr/cvw141 |
| DOI: |
10.1093/cvr/cvw141 |
| Availability: |
http://cardiovascres.oxfordjournals.org/cgi/content/short/111/suppl_1/S84; https://doi.org/10.1093/cvr/cvw141 |
| Rights: |
Copyright (C) 2016, European Society of Cardiology |
| Accession Number: |
edsbas.D6A02A3D |
| Database: |
BASE |