| Title: |
MALDI-TOF Mass Spectrometry Revealed Significant Lipid Variations in Follicular Fluid and Somatic Follicular Cells but Not in Enclosed Oocytes between the Large Dominant and Small Subordinate Follicles in Bovine Ovary |
| Authors: |
Bertevello, Priscila Silvana; Teixeira-Gomes, Ana-Paula; Labas, Valérie; Cordeiro, Luiz; Blache, Marie-Claire; Papillier, Pascal; Singina, Galina; Uzbekov, Rustem; Maillard, Virginie; Uzbekova, Svetlana |
| Contributors: |
Physiologie de la reproduction et des comportements Nouzilly (PRC); Institut Français du Cheval et de l'Equitation Saumur (IFCE)-Université de Tours (UT); NEOLAiA European University = Université Européenne NEOLAÏA (NEOLAiA)-NEOLAiA European University = Université Européenne NEOLAÏA (NEOLAiA)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE); Infectiologie et Santé Publique (ISP); Université de Tours (UT); NEOLAiA European University = Université Européenne NEOLAÏA (NEOLAiA)-NEOLAiA European University = Université Européenne NEOLAÏA (NEOLAiA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE); Chirurgie et Imagerie pour la Recherche et l'Enseignement Nouzilly (Plate-forme CIRE); Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE); L.K. Ernst Institute of Animal Husbandry; NEOLAiA European University = Université Européenne NEOLAÏA (NEOLAiA) |
| Source: |
ISSN: 1661-6596. |
| Publisher Information: |
CCSD; MDPI |
| Publication Year: |
2020 |
| Collection: |
Institut National de la Recherche Agronomique: ProdINRA |
| Subject Terms: |
extracellular vesicles; follicular cells; follicular fluid; oocyte; mass spectrometry imaging; lipid fingerprints; ovarian follicles; [SDV]Life Sciences [q-bio] |
| Description: |
International audience ; Lipid metabolism in ovarian follicular cells supports the preparation of an enclosed oocyte to ovulation. We aimed to compare lipid composition of a dominant large follicle (LF) and subordinated small follicles (SFs) within the same ovaries. Mass spectrometry imaging displayed the differences in the distribution of several lipid features between the different follicles. Comparison of lipid fingerprints between LF and SF by Matrix Assisted Laser Desorption/Ionisation Time-Of-Flight (MALDI-TOF) mass spectrometry revealed that in the oocytes, only 8 out of 468 detected lipids (1.7%) significantly changed their abundance (p < 0.05, fold change > 2). In contrast, follicular fluid (FF), granulosa, theca and cumulus cells demonstrated 55.5%, 14.9%, 5.3% and 9.8% of significantly varied features between LF and SF, respectively. In total, 25.2% of differential lipids were identified and indicated potential changes in membrane and signaling lipids. Tremendous changes in FF lipid composition were likely due to the stage specific secretions from somatic follicular cells that was in line with the differences observed from FF extracellular vesicles and gene expression of candidate genes in granulosa and theca cells between LF and SF. In addition, lipid storage in granulosa and theca cells varied in relation to follicular size and atresia. Differences in follicular cells lipid profiles between LF and SF may probably reflect follicle atresia degree and/or accumulation of appropriate lipids for post-ovulation processes as formation of corpus luteum. In contrast, the enclosed oocyte seems to be protected during final follicular growth, likely due in part to significant lipid transformations in surrounding cumulus cells. Therefore, the enclosed oocyte could likely keep lipid building blocks and energy resources to support further maturation and early embryo development. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| Relation: |
info:eu-repo/semantics/altIdentifier/pmid/32932995; PUBMED: 32932995; WOS: 000582039900001 |
| DOI: |
10.3390/ijms21186661 |
| Availability: |
https://hal.inrae.fr/hal-02959768; https://hal.inrae.fr/hal-02959768v1/document; https://hal.inrae.fr/hal-02959768v1/file/Bertevello_IJMS2020.pdf; https://doi.org/10.3390/ijms21186661 |
| Rights: |
https://creativecommons.org/licenses/by/4.0/ ; info:eu-repo/semantics/OpenAccess |
| Accession Number: |
edsbas.D6DAF811 |
| Database: |
BASE |