| Title: |
Ultrasensitive interferons quantification reveals different cytokine profile secretion in inflammatory myopathies and can serve as biomarkers of activity in dermatomyositis |
| Authors: |
Bolko, Loïs; Anquetil, Céline; Llibre, Alba; Maillard, Solène; Amelin, Damien; Dorgham, Karim; Bondet, Vincent; Landon-Cardinal, Océane; Toquet, Ségolène; Mariampillai, Kuberaka; Malatre, Samuel; Mahoudeau, Alexandrine; Hervier, Baptiste; Rodero, Mathieu P; Gorochov, Guy; Duffy, Darragh; Benveniste, Olivier; Allenbach, Yves |
| Contributors: |
Hôpital Maison Blanche; Hôpital universitaire Robert Debré Reims (CHU Reims); CHU Pitié-Salpêtrière AP-HP; Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU); Institut de Myologie; Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Association française contre les myopathies (AFM-Téléthon)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS); Immunologie Translationnelle - Translational Immunology lab; Institut Pasteur Paris (IP)-Université Paris Cité (UPCité); Centre d'Immunologie et des Maladies Infectieuses (CIMI Paris); Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS); Université de Montréal (UdeM); Centre Hospitalier de l'Université de Montréal (CHUM); Laboratoire de Chimie et de Biochimie Pharmacologiques et Toxicologiques (LCBPT - UMR 8601); Institut de Chimie - CNRS Chimie (INC-CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité) |
| Source: |
ISSN: 1664-3224. |
| Publisher Information: |
CCSD; Frontiers |
| Publication Year: |
2025 |
| Collection: |
Inserm: HAL (Institut national de la santé et de la recherche médicale) |
| Subject Terms: |
Anti-synthetase syndrom; biomarker; dermatomyositis; immune mediated necrotizing myopathie; inclusion body myositis; interferon; MESH: Humans; MESH: Dermatomyositis; MESH: Interferons; MESH: Severity of Illness Index; MESH: Biomarkers; MESH: Male; MESH: Female; MESH: Middle Aged; MESH: Myositis; MESH: Adult; MESH: Aged; MESH: Cytokines; [SDV]Life Sciences [q-bio] |
| Description: |
International audience ; Objective The objective of this study was to evaluate the presence of different types of interferon in idiopathic inflammatory myopathies (IIM) and their subgroups using ultrasensitive cytokine detection techniques (SIMOA) and to assess their potential as activity biomarkers. Methods Disease activity was measured at the time of serum collection and assessed by manual muscle testing eight (MMT8 score 0-150), muscle enzymes to calculate the Physician Global Assessment (PGA) (0-10). Patients were classified as active if PGA>5.Serum IFN-α and IFN-γ levels was measured using the single molecule array (SIMOA) technique. Serum IFN-β level was measured by Elisa. Correlation between IFN levels and disease activity were performed. Results We included 242 IIM patients and found a good correlation between type I Interferon (IFN) and dermatomyositis disease activity. IFN-α and IFN-β was highly correlated with disease activity (r=0.76 and r=0,58). To evaluate whether the different types of Interferons could serve as biomarkers of activity, we generated ROC curves. Patients with active DM had a higher median IFN-α level (0.49 pg/ml [0.1-3.7]) compared with non-active patients (0.03 pg/ml [0.01-0.07] p |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| Relation: |
info:eu-repo/semantics/altIdentifier/pmid/40013143; PUBMED: 40013143; PUBMEDCENTRAL: PMC11861187 |
| DOI: |
10.3389/fimmu.2025.1529582 |
| Availability: |
https://pasteur.hal.science/pasteur-04989745; https://pasteur.hal.science/pasteur-04989745v1/document; https://pasteur.hal.science/pasteur-04989745v1/file/fimmu-1-1529582.pdf; https://doi.org/10.3389/fimmu.2025.1529582 |
| Rights: |
https://creativecommons.org/licenses/by/4.0/ ; info:eu-repo/semantics/OpenAccess |
| Accession Number: |
edsbas.D6FD4D1D |
| Database: |
BASE |