| Title: |
Low agreement and frequent invalid controls in two SARS-CoV-2 T-cell assays in people with compromised immune function |
| Authors: |
Audigé, Annette; Amstutz, Alain; Schuurmans, Macé M; Amico, Patrizia; Braun, Dominique L; Stoeckle, Marcel P; Hasse, Barbara; Hage, René; Damm, Dominik; Tamm, Michael; Mueller, Nicolas J; Günthard, Huldrych F; Koller, Michael T; Schönenberger, Christof M; Griessbach, Alexandra; Labhardt, Niklaus D; Kouyos, Roger D; Trkola, Alexandra; Huber, Michael; Kusejko, Katharina; Bucher, Heiner C; Abela, Irene A; Briel, Matthias; Chammartin, Frédérique; Speich, Benjamin; Swiss HIV Cohort Study; Swiss Transplant Cohort Study |
| Source: |
Audigé, Annette; Amstutz, Alain; Schuurmans, Macé M; Amico, Patrizia; Braun, Dominique L; Stoeckle, Marcel P; Hasse, Barbara; Hage, René; Damm, Dominik; Tamm, Michael; Mueller, Nicolas J; Günthard, Huldrych F; Koller, Michael T; Schönenberger, Christof M; Griessbach, Alexandra; Labhardt, Niklaus D; Kouyos, Roger D; Trkola, Alexandra; Huber, Michael; Kusejko, Katharina; Bucher, Heiner C; Abela, Irene A; Briel, Matthias; Chammartin, Frédérique; Speich, Benjamin; Swiss HIV Cohort Study; Swiss Transplant Cohort Study; et al (2025). Low agreement and frequent invalid controls in two SARS-CoV-2 T-cell assays in people with compromised immune function. PLoS ONE, 20(1):e0317965. |
| Publisher Information: |
Public Library of Science (PLoS) |
| Publication Year: |
2025 |
| Collection: |
University of Zurich (UZH): ZORA (Zurich Open Repository and Archive |
| Subject Terms: |
Clinic for Infectious Diseases; Institute of Medical Virology; 570 Life sciences; biology; 610 Medicine & health |
| Description: |
T-cell response plays an important role in SARS-CoV-2 immunogenicity. For people living with HIV (PWH) and solid organ transplant (SOT) recipients there is limited evidence on the reliability of commercially available T-cell tests. We assessed 173 blood samples from 81 participants (62 samples from 35 PWH; 111 samples from 46 SOT recipients [lung and kidney]) with two commercial SARS-CoV-2 Interferon-γ (IFN-γ) release assays (IGRA; SARS-CoV-2 IGRA by Euroimmun, and IGRA SARS-CoV-2 by Roche). The reliability between the tests was judged as low (Cohen's kappa [κ] = 0.20; overall percent agreement [OPA] = 66%). A high proportion of tests were invalid (22% Euroimmun; 8% Roche). When excluding these invalid tests, the agreement was higher (κ = 0.43; OPA = 90%). The low reliability between the two T-cell tests indicates that results should be interpreted with caution in SOT recipients and PWH and that SARS-CoV-2 T-cell tests need to be optimized and further validated for use in vulnerable patient populations. |
| Document Type: |
article in journal/newspaper |
| File Description: |
application/pdf |
| Language: |
English |
| ISSN: |
1932-6203 |
| Relation: |
https://www.zora.uzh.ch/id/eprint/275961/1/journal.pone.0317965.pdf; info:pmid/39854310; urn:issn:1932-6203 |
| DOI: |
10.1371/journal.pone.0317965 |
| Availability: |
https://www.zora.uzh.ch/id/eprint/275961/; https://www.zora.uzh.ch/id/eprint/275961/1/journal.pone.0317965.pdf; https://doi.org/10.1371/journal.pone.0317965 |
| Rights: |
info:eu-repo/semantics/openAccess ; Creative Commons: Attribution 4.0 International (CC BY 4.0) ; http://creativecommons.org/licenses/by/4.0/ |
| Accession Number: |
edsbas.D73F4607 |
| Database: |
BASE |