| Title: |
Schistosomes induce regulatory features in human and mouse CD1d(hi) B cells: inhibition of allergic inflammation by IL-10 and regulatory T cells. |
| Authors: |
Luciën E P M van der Vlugt; Lucja A Labuda; Arifa Ozir-Fazalalikhan; Ellen Lievers; Anouk K Gloudemans; Kit-Yeng Liu; Tom A Barr; Tim Sparwasser; Louis Boon; Ulysse Ateba Ngoa; Eliane Ngoune Feugap; Ayola A Adegnika; Peter G Kremsner; David Gray; Maria Yazdanbakhsh; Hermelijn H Smits |
| Source: |
PLoS ONE, Vol 7, Iss 2, p e30883 (2012) |
| Publisher Information: |
Public Library of Science (PLoS) |
| Publication Year: |
2012 |
| Collection: |
Directory of Open Access Journals: DOAJ Articles |
| Subject Terms: |
Medicine; Science |
| Description: |
Chronic helminth infections, such as schistosomes, are negatively associated with allergic disorders. Here, using B cell IL-10-deficient mice, Schistosoma mansoni-mediated protection against experimental ovalbumin-induced allergic airway inflammation (AAI) was shown to be specifically dependent on IL-10-producing B cells. To study the organs involved, we transferred B cells from lungs, mesenteric lymph nodes or spleen of OVA-infected mice to recipient OVA-sensitized mice, and showed that both lung and splenic B cells reduced AAI, but only splenic B cells in an IL-10-dependent manner. Although splenic B cell protection was accompanied by elevated levels of pulmonary FoxP3(+) regulatory T cells, in vivo ablation of FoxP3(+) T cells only moderately restored AAI, indicating an important role for the direct suppressory effect of regulatory B cells. Splenic marginal zone CD1d(+) B cells proved to be the responsible splenic B cell subset as they produced high levels of IL-10 and induced FoxP3(+) T cells in vitro. Indeed, transfer of CD1d(+) MZ-depleted splenic B cells from infected mice restored AAI. Markedly, we found a similarly elevated population of CD1d(hi) B cells in peripheral blood of Schistosoma haematobium-infected Gabonese children compared to uninfected children and these cells produced elevated levels of IL-10. Importantly, the number of IL-10-producing CD1d(hi) B cells was reduced after anti-schistosome treatment. This study points out that in both mice and men schistosomes have the capacity to drive the development of IL-10-producing regulatory CD1d(hi) B cells and furthermore, these are instrumental in reducing experimental allergic inflammation in mice. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| Relation: |
http://europepmc.org/articles/PMC3275567?pdf=render; https://doaj.org/toc/1932-6203; https://doaj.org/article/95afc997c7a54b098fe0b5c3e0f0d247 |
| DOI: |
10.1371/journal.pone.0030883 |
| Availability: |
https://doi.org/10.1371/journal.pone.0030883; https://doaj.org/article/95afc997c7a54b098fe0b5c3e0f0d247 |
| Accession Number: |
edsbas.D7D2408C |
| Database: |
BASE |