| Title: |
Red light-emitting short Mango-based system enables tracking a mycobacterial small noncoding RNA in infected macrophages |
| Authors: |
Bychenko, Oksana S; Khrulev, Alexei A; Svetlova, Julia I; Tsvetkov, Vladimir B; Kamzeeva, Polina N; Skvortsova, Yulia V; Tupertsev, Boris S; Ivanov, Igor A; Aseev, Leonid V; Khodarovich, Yuriy M; Belyaev, Evgeny S; Kozlovskaya, Liubov I; Zatsepin, Timofei S; Azhikina, Tatyana L; Varizhuk, Anna M; Aralov, Andrey V |
| Contributors: |
Russian Foundation for Basic Research; Russian Science Foundation; Ministry of Science and Higher Education of the Russian Federation |
| Source: |
Nucleic Acids Research ; volume 51, issue 6, page 2586-2601 ; ISSN 0305-1048 1362-4962 |
| Publisher Information: |
Oxford University Press (OUP) |
| Publication Year: |
2023 |
| Description: |
Progress in RNA metabolism and function studies relies largely on molecular imaging systems, including those comprising a fluorogenic dye and an aptamer-based fluorescence-activating tag. G4 aptamers of the Mango family, typically combined with a duplex/hairpin scaffold, activate the fluorescence of a green light-emitting dye TO1-biotin and hold great promise for intracellular RNA tracking. Here, we report a new Mango-based imaging platform. Its key advantages are the tunability of spectral properties and applicability for visualization of small RNA molecules that require minimal tag size. The former advantage is due to an expanded (green-to-red-emitting) palette of TO1-inspired fluorogenic dyes, and the truncated duplex scaffold ensures the latter. To illustrate the applicability of the improved platform, we tagged Mycobacterium tuberculosis sncRNA with the shortened aptamer-scaffold tag. Then, we visualized it in bacteria and bacteria-infected macrophages using the new red light-emitting Mango-activated dye. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| DOI: |
10.1093/nar/gkad100 |
| Availability: |
https://doi.org/10.1093/nar/gkad100; https://academic.oup.com/nar/article-pdf/51/6/2586/49761672/gkad100.pdf |
| Rights: |
https://creativecommons.org/licenses/by/4.0/ |
| Accession Number: |
edsbas.D9BB5DF6 |
| Database: |
BASE |