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Evidence incriminating Anopheles maculatus as a potential vector of Plasmodium knowlesi and Plasmodium inui

Title: Evidence incriminating Anopheles maculatus as a potential vector of Plasmodium knowlesi and Plasmodium inui
Authors: Boni F. Sebayang; Bram van de Straat; Ahadi Kurniawan; Triwibowo A. Garjito; Adzkia M. Haq; Jacob A. F. Westaway; Inke N. D. Lubis; Matthew J. Grigg; Tanya L. Russell; Thomas R. Burkot
Source: Scientific Reports, Vol 16, Iss 1 (2026)
Publisher Information: Nature Portfolio
Publication Year: 2026
Collection: Directory of Open Access Journals: DOAJ Articles
Subject Terms: Anopheles maculatus; North sumatra; Zoonotic malaria; Plasmodium knowlesi; Medicine; Science
Description: In Indonesia, 29 anopheline species are vectors of human malarias. Many of these morphologically indistinguishable vectors are in species complexes requiring molecular analysis to identify. Data on vectors in North Sumatra remain limited. This study investigated mosquitoes of the Maculatus Subgroup in Ujung Bandar Village, Langkat Regency, to identify them and their potential roles in malaria transmission. Mosquitoes collected via human landing catches (July 2022 – June 2023) were analysed using ITS2 PCR assays and a phylogenetic analysis. Screening for Plasmodium DNA was performed on mosquito heads and thoraces using RT-qPCR targeting the 18S rRNA, followed by species-specific nested PCR. All 234 specimens morphologically identified An. maculatus s.l. were confirmed as An. maculatus s.s., being genetically consistent with populations from Indonesia and mainland Southeast Asia. Four (1.7%; 95% CI: 0.5–4.3%) An. maculatus specimens were positive for Plasmodium genus DNA, with one specimen positive for the DNA of both Plasmodium knowlesi and Plasmodium inui. This represents the first detection of zoonotic malaria parasite DNA in wild-caught An. maculatus. These findings suggest that An. maculatus may be a potential vector of both zoonotic and human malarias in North Sumatra.
Document Type: article in journal/newspaper
Language: English
Relation: https://doi.org/10.1038/s41598-026-35946-z; https://doaj.org/toc/2045-2322; https://doaj.org/article/37dab22169ac40d49208915b71b92885
DOI: 10.1038/s41598-026-35946-z
Availability: https://doi.org/10.1038/s41598-026-35946-z; https://doaj.org/article/37dab22169ac40d49208915b71b92885
Accession Number: edsbas.D9DC9483
Database: BASE