| Title: |
Single-cell transcriptomics identifies aberrant glomerular angiogenic signalling in the early stages of WT1 kidney disease |
| Authors: |
Chandler, Jennifer C.; Jafree, Daniyal J.; Malik, Saif; Pomeranz, Gideon; Ball, Mary; Kolatsi-Joannou, Maria; Piapi, Alice; Mason, William J.; Benest, Andrew V.; Bates, David O.; Letunovska, Aleksandra; Al-Saadi, Reem; Rabant, Marion; Boyer, Olivia; Pritchard-Jones, Kathy; Winyard, Paul J.; Mason, Andrew S.; Woolf, Adrian S.; Waters, Aoife M.; Long, David A. |
| Publisher Information: |
Wiley |
| Publication Year: |
2024 |
| Collection: |
University of Pretoria: UPSpace |
| Subject Terms: |
Vascular endothelial growth factor-A; WT1 glomerulopathy; Wilms tumour 1 (WT1); Single-cell transcriptomics; Podocyte; Neuropilin-1; Glomerular endothelium; Childhood glomerular disease; Adrenomedullin; SDG-03: Good health and well-being |
| Description: |
DATA AVAILABILITY STATEMENT : Primary sequencing data reported in this paper are available on NCBI Sequence Read Archive (SRA PRJNA928337). Processed data for scRNA-seq experiments are available on Zenodo at DOI:10.5281/zenodo.7565867. The codes generated during this study are available at Github repository https://github.com/daniyal-jafree1995/collaborations. All other unique reagents used in this study are available from the corresponding author on reasonable request. ; SUPPLEMENTARY MATERIALS AND METHODS : FIGURE S1. Supporting data for single-cell RNA seq analyses. FIGURE S2. Characterisation of primary cell lines. FIGURE S3. Isolation of podocytes from scRNA-seq data from additional murine models of glomerular disease for cross-disease comparison. FIGURE S4. Dysregulated genes shown by individual sample for each human glomerular pathology (source Nephroseq). TABLE S1. Differentially expressed genes in glomerular cells in Wt1R394W/+ mice. TABLE S2. Podocyte differentially expressed genes that are conserved across murine glomerular scRNA-seq datasets or unique to Wt1R394W/+. TABLE S3. Podocyte differentially expressed genes classified in ‘GO:0002376 – immune system process’ across murine glomerular scRNA-seq datasets. ; Please read abstract in the article. ; LifeArc/Great Ormond Street Children's Charity; Medical Research Centre; Kidney Research UK; British Heart Foundation; Diabetes UK; Wellcome Trust. ; https://onlinelibrary.wiley.com/journal/10969896 ; hj2024 ; Medical Oncology ; SDG-03:Good heatlh and well-being |
| Document Type: |
article in journal/newspaper |
| File Description: |
application/pdf; application/vnd.openxmlformats-officedocument.spreadsheetml.sheet |
| Language: |
English |
| Relation: |
http://hdl.handle.net/2263/98857 |
| DOI: |
10.1002/path.6339 |
| Availability: |
http://hdl.handle.net/2263/98857; https://doi.org/10.1002/path.6339 |
| Rights: |
© 2024 The Author(s). The Journal of Pathology published by John Wiley & Sons Ltd on behalf of The Pathological Society of Great Britain and Ireland. This is an open access article under the terms of the Creative Commons Attribution License. |
| Accession Number: |
edsbas.DA363986 |
| Database: |
BASE |