| Title: |
Description of the vCircTrappist process. |
| Authors: |
Alexis S. Chasseur; Maxime Bellefroid; Mathilde Galais; Meijiao Gong; Pierre Lombard; Sarah Mathieu; Amandine Pecquet; Estelle Plant; Camille Ponsard; Laure Vreux; Carlo Yague-Sanz; Benjamin G. Dewals; Nicolas A. Gillet; Benoît Muylkens; Carine M. Van Lint; Damien Coupeau |
| Publication Year: |
2025 |
| Subject Terms: |
Medicine; Microbiology; Genetics; Molecular Biology; Evolutionary Biology; Immunology; Infectious Diseases; Virology; Biological Sciences not elsewhere classified; +non%22">xlink "> non; unconventional splicing mechanisms; sanger sequencings validated; recent attention focused; obtained novel insights; initially anticipated due; identify backsplicing events; human pathogens belonging; existing datasets encompassing; conserved eukaryotic patterns; coding rnas play; viral infection cycles; viral genomes include; unique viral characteristics; rna sequencing data; developed bioinformatic tool; various viral families; bioinformatic pipeline tailored; families |
| Description: |
(A) Mapping on the viral genome. The whole set of reads is mapped to the viral genome using BWA. (B) Sorting the spliced reads. The reads that present a splicing signature were recovered while the others were deleted. (C) Sorting the backsplice junction spanning reads. (D) Read characterization. The reads were sorted according to their features such as their splicing pattern or the strand they were originating from. The reads that correspond to a canonical U2 splicing pattern are depicted in black, while the non-canonical splicing reads are depicted in orange. The curves above and below the graph represent the 20 most abundant circRNA candidates found in the dataset. (A-D) On the left panels, the genome structure of the viral bacmid used for the transfection is depicted above the coverage plots. The genome is composed of two terminal repeats. The internal repeats were removed by the insertion of the bacmid cassette. The unique regions, short and long, are depicted. The X axis indicates the position on the viral genome (bp). The Y axis represent the normalized coverage. The normalization was made on the number of reads mapped at a specific locus divided by the total number of reads mapping on the viral genome, multiplied by one billion. On the right panels, the genome is depicted in black while the reads are depicted in green (linear splicing reads), blue (non-spliced reads) and black or orange (canonical and non-canonical backsplice junction spanning reads, respectively). |
| Document Type: |
still image |
| Language: |
unknown |
| Relation: |
https://figshare.com/articles/figure/Description_of_the_vCircTrappist_process_/30105335 |
| DOI: |
10.1371/journal.ppat.1013448.g001 |
| Availability: |
https://doi.org/10.1371/journal.ppat.1013448.g001; https://figshare.com/articles/figure/Description_of_the_vCircTrappist_process_/30105335 |
| Rights: |
CC BY 4.0 |
| Accession Number: |
edsbas.DB6E4DD6 |
| Database: |
BASE |